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KLF9 Antibody (701888) in WB
Western blot analysis was performed on extracts (30 µg of lysate) of SK-OV-3 (Lane 1), HEL (Lane 2), KARPAS 299 (Lane 3), HEK-293 (Lane 4), U-87 MG (Lane 5), Jurkat (Lane 6), Mouse Liver (Lane 7), Rat Liver (Lane 8). The blots were probed with KLF9 Recombinant Rabbit Monoclonal Antibody (Product # 701888, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 27 kDa band corresponding to KLF9 was observed across c... View More
产品信息
701888
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
5H16L7
抗原
Peptides corresponding to Human KLF9 (aa 34-49, 85-98)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2633037
产品详细信息
This antibody is predicted to react with Bovine, Pig and Monkey.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
KLF-9 is a transcription factor belonging to the Sp 1 C2H2 type zinc finger family. It binds to GC box elements located in the promoter. This binding of the encoded protein to a single GC box inhibits mRNA expression while binding to tandemly repeated GC box elements activates transcription. KLF-9 serves as a key transcriptional regulator for uterine endometrial cell proliferation, adhesion, and differentiation both during pregnancy and tumorigenesis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: basic transcription element binding protein 1; Basic transcription element-binding protein 1; BTE-binding protein 1; GC-box-binding protein 1; Krueppel-like factor 9; Kruppel-like factor 9; Transcription factor BTEB1
基因别名: 2310051E17Rik; AA589643; BTEB; BTEB-1; BTEB1; ENSMUSG00000055440; Gm9971; KLF9
UniProt ID: (Human) Q13886, (Rat) Q01713
Entrez Gene ID: (Human) 687, (Mouse) 16601, (Rat) 117560
DNA binding
transcription factor activity, sequence-specific DNA binding
metal ion binding
RNA polymerase II regulatory region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
nucleic acid binding
C2H2 zinc finger transcription factor
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
circadian rhythm
negative regulation of keratinocyte proliferation
cellular response to cortisol stimulus
cellular response to thyroid hormone stimulus
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
embryo implantation
positive regulation of transcription from RNA polymerase II promoter
rhythmic process
progesterone receptor signaling pathway
cellular response to peptide
