Invitrogen

KLRB1 Monoclonal Antibody (3.2.3)

产品信息

MANK07

应用

建议稀释比

已发表文章

免疫组化 (IHC)

Assay-dependent

查看4篇已发表文章 4篇已发表文章

免疫细胞化学 (ICC/IF)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

5 µL/sample

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产品规格

种属反应

Rat

已发表种属

Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

3.2.3

抗原

Rat 24.5 kD cell surface protein known as NKR-P1

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_223597

产品详细信息

MANK07 targets NKR-P1 in FACS, and IHC applications and shows reactivity with Rat samples.

The MANK07 immunogen is rat 24.5 kD cell surface protein known as NKR-P1.

MANK07 detects NKR-P1 which has a predicted molecular weight of approximately 25 kDa.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

靶标信息

KLRB1 (Killer cell lectin like receptor subfamily B, member 1, CD-161) protein is classified as a type II membrane protein because it has an external C terminus. KLRB1 cell surface antigen is expressed by almost all NK cells and in a small subset of CD3+ve T cells. KLRB1 is a homodimeric cell surface protein, comprising two chains with molecular weights ranging from 40-44kDa. KLRB1 plays an inhibitory role on natural killer (NK) cells. Activation of KLRB1 leads to acid sphingomyelnase/SMPD1 stimulation and an increase in intracellular ceramide. Moreover, there is also an activation of AKT1/PKB and RPS6KA1/RSK1 kinase stimulation, and T cell proliferation by anti-CD3. KLRB1 acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope and to the N-acetyllactosamine epitope. KLRB1 also binds to CLEC2D/LLT1 as a ligand, and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Protease-activated receptor 1 activation is necessary for monocyte chemoattractant protein 1-dependent leukocyte recruitment in vivo.

The Journal of experimental medicine

Chen D,Carpenter A,Abrahams J,Chambers RC,Lechler RI,McVey JH,Dorling A

MANK07 was used in immunocytochemistry to study the role of PAR activation in vivo to initiate MCP-dependent leukocyte recruitment

Mon Aug 04 00:00:00 UTC 2008

A transmembrane-anchored rat RAE-1-like transcript as a ligand for NKR-P2, the rat ortholog of human and mouse NKG2D.

European journal of immunology

Savithri B,Khar A

MANK07 was used in flow cytometry to identify the ligand for the rat ortholog of human and mouse NKG2D

Sun Jan 01 00:00:00 UTC 2006

Target cell induced activation of NK cells in vitro: cytokine production and enhancement of cytotoxic function.

Cancer immunology, immunotherapy : CII

Das S,Varalakshmi C,Kumari AL,Patel M,Khar A

MANK07 was used in flow cytometry to investigate the changes of cytokine expression in NK cells after interacting with tumor cells

Mon Oct 01 00:00:00 UTC 2001

Efficient suicide gene therapy of transduced and distant untransduced ovary tumors is correlated with significant increase of intratumoral T and NK cells.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Nagy HJ,Panis Y,Fabre M,Engelmann C,Soubrane O,Houssin D,Klatzmann D

MANK07 was used in immunohistochemistry to study the mechanism for the suicide gene therapy of ovary tumors

Sun Oct 01 00:00:00 UTC 2000

Target-cell-induced anergy in natural killer cells: suppression of cytotoxic function.

Cancer immunology, immunotherapy : CII

Das S,Varalakshmi C,Khar A

MANK07 was used in flow cytometry to investigate the cytotoxic functions of natural killer cells

Mon May 01 00:00:00 UTC 2000

Interleukin-2-induced nitric oxide synthase and nuclear factor-kappaB activity in activated natural killer cells and the production of interferon-gamma.

Scandinavian journal of immunology

Jyothi MD,Khar A

MANK07 was used in flow cytometry to investigate the role of interleukin 2 in the regulation of natural killer cell function

Tue Aug 01 00:00:00 UTC 2000

Eradication of rat malignant gliomas by retroviral-mediated, in vivo delivery of the interleukin 4 gene.

Cancer research

Benedetti S,Bruzzone MG,Pollo B,DiMeco F,Magrassi L,Pirola B,Cirenei N,Colombo MP,Finocchiaro G

MANK07 was used in immunohistochemistry to evaluate the interleukin 4 gene therapy of rat malignant gliomas

Mon Feb 01 00:00:00 UTC 1999

Lymphocyte subpopulations in lymphoid organs of rats after acute resistance exercise.

Medicine and science in sports and exercise

Mastro AM,Schlosser DA,Grove DS,Lincoski C,Pishak SA,Gordon S,Kraemer WJ

MANK07 was used in flow cytometry to investigate the changes of lymphocyte subpopulations in vivo with acute resistance exercise

Fri Jan 01 00:00:00 UTC 1999

Administration of anti-IL-12 antibody in vivo inhibits rejection of a rat histiocytoma and suppresses cytokine response in a tumour-bearing host.

Immunology

Rao KL,Varalakshmi C,Ali AM,Khar A

MANK07 was used in flow cytometry to investigate the effect of interleukin 12 inhibition on tumor rejection

Sat Nov 01 00:00:00 UTC 1997

Phospholipase A2-mediated inflammation induces regression of malignant gliomas.

Cancer letters

Goddard DH,Bomalaski JS,Lipper S,Shorr RG,Clark MA

MANK07 was used in immunohistochemistry to investigate the effect of phospholipase A2 on glioma inhibition in vivo

Fri Apr 19 00:00:00 UTC 1996

Linomide inhibits angiogenesis, growth, metastasis, and macrophage infiltration within rat prostatic cancers.

Cancer research

Vukanovic J,Isaacs JT

MANK07 was used in immunohistochemistry to study the role of mitochondrial pathway in neutrophil survival modulated by IgE in asthma

Sat Apr 01 00:00:00 UTC 1995

Natural killer cell activation-associated induction of target cell DNA fragmentation in a spontaneously regressing rat histiocytoma.

Immunology

Bright JJ,Kausalya S,Khar A

MANK07 was used in flow cytometry to investigate the effect of natural killer cells on apoptosis in a regressing histiocytoma

Tue Aug 01 00:00:00 UTC 1995

生物信息学

蛋白别名: Antigen 3.2.3; CD161 antigen-like family member A; CD161 antigen-like family member B; CD161a; CD161b; Immunoreceptor NKR-P1E; Killer cell lectin-like receptor subfamily B member 1; Killer cell lectin-like receptor subfamily B member 1A; killer cell lectin-like receptor subfamily B member 1B; Killer cell lectin-like receptor subfamily B member 1B allele A; killer cell lectin-like receptor subfamily B member 1B allele C; killer cell lectin-like receptor subfamily B member 1F; Killer cell lectin-like receptor subfamily B member 1G; klrb1b {ECO:0000312|EMBL:AAQ11375.1}; klrb1b {ECO:0000312|RGD:2975}; MGC138614; natural killer cell receptor; Natural killer cell surface protein NKR-P1B allele RNK/SD/BN/F344; natural killer cell surface protein NKR-P1B allele TO; Natural killer cell surface protein NKR-P1G; Natural killer cell surface protein P1-3.2.3; Natural killer lectin-like receptor 1E; natural killer lectin-like receptor protein 1 e; NKR-P1 3.2.3; NKR-P1A

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基因别名: Klrb1; Klrb1a; Klrb1b; Klrb1d; Klrb1f; Klrb1g; Klrb6; NKR-P1A; Nkr-p1b; Nkr-p1e; NKR-P1G; Nkrp-1e; Nkrp1a; Nkrp1b; Nkrp1e; Nkrp1g

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UniProt ID: (Rat) Q0ZUP0, (Rat) P27471, (Rat) A4KWA1

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Entrez Gene ID: (Rat) 689817, (Rat) 362443, (Rat) 25192

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功能

receptor activity carbohydrate binding protein homodimerization activity