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Ki-67 Antibody (PA1-21520) in ICC/IF
Immunofluorescence analysis of Ki67 was performed using 70% confluent log phase HeLa cells serum starved for 36 hours followed by serum release for 6 hrs. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with Ki67 Polyclonal Antibody (Product # PA1-21520) at 10 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Seco... View More
产品信息
PA1-21520
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide from the human Ki-67 antigen.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS with 0.2% BSA
内含物
0.1% sodium azide
保存条件
4° C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_559806
产品详细信息
Recommended positive controls: Tonsil or Breast carcinoma.
Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM citrate buffer, pH6.0, for 10-20 minutes, followed by coolong at room temperature for 20 minutes.
靶标信息
Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2
基因别名: D630048A14Rik; Ki-67; Ki67; KIA; MIB-; MIB-1; MKI67; PPP1R105
UniProt ID: (Human) P46013, (Mouse) E9PVX6
Entrez Gene ID: (Human) 4288, (Rat) 291234, (Mouse) 17345
