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Kinesin 5A Antibody (PA1-642) in ICC/IF
Immunofluorescence analysis of Kinesin 5A was done on 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Kinesin 5A Rabbit Polyclonal Antibody (Product # PA1-642) at 1 µg/mL in 1% BSA and incubated for 3 hours at room tem... View More
产品信息
PA1-642
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Bovine
已发表种属
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic Peptide: C(1007) G Y E A E D Q A K L F P L H Q E T A A S(1027)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2132216
产品详细信息
PA1-642 detects kinesin 5A from mouse, human, and bovine samples. This antibody is specific for kinesin 5A and does not detect other kinesin isotypes.
PA1-642 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~110 kDa protein representing kinesin 5A from mouse neuronal cell lysate and human retinal extracts.
The PA1-642 immunizing peptide corresponds to amino acid residues 1007-1027 from mouse kinesin 5A. This sequence is completely conserved in human. This peptide (Cat. # PEP-197) is available for use in neutralization and control experiments.
靶标信息
This gene encodes a member of the kinesin family of proteins. Members of this family are part of a multisubunit complex that functions as a microtubule motor in intracellular organelle transport. Mutations in this gene cause autosomal dominant spastic paraplegia 10.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: KIF5A variant protein; Kinesin heavy chain isoform 5A; Kinesin heavy chain neuron-specific 1; kinesin, heavy chain, neuron-specific; Neuronal kinesin heavy chain; NKHC
基因别名: BOS_5453; D10Bwg0738e; D12S1889; Khc; Kiaa4086; Kif5; KIF5A; Kns; mKIAA4086; MY050; NKHC; NKHC1; SPG10
UniProt ID: (Human) Q12840, (Mouse) P33175
Entrez Gene ID: (Human) 3798, (Bovine) 511273, (Mouse) 16572
