Rockland

Kmo Polyclonal Antibody

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Kmo Antibody (600-401-J26) in WB

Western Blot of Rabbit anti-KMO antibody. Lane 1: Brain Extract. Load: 10 µg per lane. Primary antibody: KMO antibody at 1µg/mL for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 58 kDa for KMO. Other band(s): Other bands at lower molecular weights. These bands are all specifically blocked by KMO peptide.

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产品信息

600-401-J26

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

免疫组化 (IHC)

1:100-1:500

酶联免疫吸附实验 (ELISA)

1:20,000-1:60,000

免疫沉淀 (IP)

1 µg/mL

产品规格

种属反应

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KMO affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding 200-300aa of the mouse KMO chain.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.07 mg/mL

纯化类型

Affinity chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 50% glycerol, 0.15M NaCl

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-KMO specific towards Kynurenine 3-monooxygenase (KMO). A BLAST analysis was used to suggest cross-reactivity with Mouse based on 100% sequence homology. Cross-reactivity with KMO from other sources has not been determined.

靶标信息

Kynurenine 3-monooxygenase (KMO; EC 1.14.13.9) is an NADPH-dependent flavin monooxygenase that catalyzes the hydroxylation of the L-tryptophan metabolite L-kynurenine to form L-3-hydroxykynurenine.

仅用于科研。不用于诊断过程。未经明确授权不得转售。