Invitrogen

Ku70/Ku80 Monoclonal Antibody (162)

Ku70/Ku80 Antibody in Immunocytochemistry (ICC/IF)

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Ku70/Ku80 Antibody (MA5-12938) in ICC/IF

Immunofluorescent analysis of Ku (p70/p80) (green) showing staining in the nucleus of MCF-7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Ku (p70/p80) monoclonal antibody (Product # MA5-12938) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 ºC in a humidi... View More

Ku70/Ku80 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Ku70/Ku80 Antibody in Flow Cytometry (Flow)

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产品信息

MA5-12938

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

1-2 µg/mL

免疫细胞化学 (ICC/IF)

1:100-1:500

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

0.5 µg/test

免疫沉淀 (IP)

查看8篇已发表文章 8篇已发表文章

染色质免疫沉淀实验 (ChIP)

查看1篇已发表文章 1篇已发表文章

凝胶阻滞分析 (GS)

1mg/mL

查看5篇已发表文章 5篇已发表文章

阻断反应 (BLOCK)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Non-human primate, Xenopus

已发表种属

Hamster, Human, Non-human primate

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

162

抗原

Human B cell nuclei from plasmacytoid 2p68 cells

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

PBS, pH 7.4, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_10980693

产品详细信息

MA5-12938 targets Ku (p70/p80) in FACS, GS, ICC/IF, and IHC (P) applications and shows reactivity with Human, Non-human primate, Mouse and Xenopus laevis samples. This antibody does not cross-react with mouse in immunofluorescent applications, but does react with mouse in FACS applications.

The MA5-12938 immunogen is human B cell nuclei from plasmacytoid 2p68 cells.

靶标信息

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008].

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Characterization of estrogen response element binding proteins as biomarkers of breast cancer behavior.

Clinical biochemistry

Kruer TL,Cummins TD,Powell DW,Wittliff JL

MA5-12938 was used in EMSA to study the prognostic value of estrogen resonse element binding proteins in breast cancer

Fri Nov 01 00:00:00 UTC 2013

Ku counteracts mobilization of PARP1 and MRN in chromatin damaged with DNA double-strand breaks.

Nucleic acids research

Cheng Q,Barboule N,Frit P,Gomez D,Bombarde O,Couderc B,Ren GS,Salles B,Calsou P

MA5-12938 was used in western blot to study the role of PARP1 and MRN proteins in the repair of DNA double strand breaks

Thu Dec 01 00:00:00 UTC 2011

The DNA repair complex Ku70/86 modulates Apaf1 expression upon DNA damage.

Cell death and differentiation

De Zio D,Bordi M,Tino E,Lanzuolo C,Ferraro E,Mora E,Ciccosanti F,Fimia GM,Orlando V,Cecconi F

MA5-12938 was used in ChIP assay to study the role of the Ku70/86 complex in modulating Apaf-1 expression upon DNA damage

Tue Mar 01 00:00:00 UTC 2011

TRF2/RAP1 and DNA-PK mediate a double protection against joining at telomeric ends.

The EMBO journal

Bombarde O,Boby C,Gomez D,Frit P,Giraud-Panis MJ,Gilson E,Salles B,Calsou P

MA5-12938 was used in immunoprecipitation to investigate the role of TRF2/RAP1 and DNA-PK at telomeric fusion

Wed May 05 00:00:00 UTC 2010

The effect of DNA-dependent protein kinase on adeno-associated virus replication.

PloS one

Choi YK,Nash K,Byrne BJ,Muzyczka N,Song S

MA5-12938 was used in immunoprecipitation to study the role of DNA-dependent protein kinase in adeno-associated virus replication

Mon Dec 20 00:00:00 UTC 2010

Accumulation of Ku80 proteins at DNA double-strand breaks in living cells.

Experimental cell research

Koike M,Koike A

MA5-12938 was used in immunocytochemistry to study the accumulation Ku80 proteins at DNA double-strand breaks in living cells

Mon Mar 10 00:00:00 UTC 2008

Loading of the nonhomologous end joining factor, Ku, on protein-occluded DNA ends.

The Journal of biological chemistry

Roberts SA,Ramsden DA

MA5-12938 was used in EMSA to study the mechanism by which the nonhomologous end joining factor Ku is loaded on protein-occluded DNA ends

Fri Apr 06 00:00:00 UTC 2007

Interplay between Ku, Artemis, and the DNA-dependent protein kinase catalytic subunit at DNA ends.

The Journal of biological chemistry

Drouet J,Frit P,Delteil C,de Villartay JP,Salles B,Calsou P

MA5-12938 was used in immunoprecipitation to study the interaction of Artemis with proteins involved of the non-homologous end joining pathway

Fri Sep 22 00:00:00 UTC 2006

Ongoing immunoglobulin class switch DNA recombination in lupus B cells: analysis of switch regulatory regions.

Autoimmunity

Liu S,Cerutti A,Casali P,Crow MK

MA5-12938 was used in EMSA to study the role of exogenous signals in ongoing immunoglobulin class switching DNA recombination in lupus B cells

Thu May 12 00:00:00 UTC 2005

Involvement of poly(ADP-ribose) polymerase-1 and XRCC1/DNA ligase III in an alternative route for DNA double-strand breaks rejoining.

The Journal of biological chemistry

Audebert M,Salles B,Calsou P

MA5-12938 was used in immunoprecipitation to study an alternative pathway for the repair of DNA double-strand breaks that involves poly(ADP-ribose) polymerase-1 and a XRCC1-DNA ligase III complex

Fri Dec 31 00:00:00 UTC 2004

Ku70/Ku80 and DNA-dependent protein kinase catalytic subunit modulate RAG-mediated cleavage: implications for the enforcement of the 12/23 rule.

The Journal of biological chemistry

Sawchuk DJ,Mansilla-Soto J,Alarcon C,Singha NC,Langen H,Bianchi ME,Lees-Miller SP,Nussenzweig MC,Cortes P

MA5-12938 was used in blocking or activating experiment to study the role of Ku70/80 and DNA-PK in regulating RAG-mediated cleavage and the significance for the 12/23 rule specificity

Fri Jul 09 00:00:00 UTC 2004

Possible anti-recombinogenic role of Bloom's syndrome helicase in double-strand break processing.

Nucleic acids research

Onclercq-Delic R,Calsou P,Delteil C,Salles B,Papadopoulo D,Amor-Guéret M

MA5-12938 was used in immunoprecipitation to study the potential anti-recombinogenic role of BLM DNA helicase in double-strand break processing

Sat Nov 01 00:00:00 UTC 2003

Biochemical evidence for Ku-independent backup pathways of NHEJ.

Nucleic acids research

Wang H,Perrault AR,Takeda Y,Qin W,Wang H,Iliakis G

MA5-12938 was used in western blot to study alternative backup pathways of non-homologous end joining that are independent of Ku

Mon Sep 15 00:00:00 UTC 2003

Differential DNA binding of Ku antigen determines its involvement in DNA replication.

DNA and cell biology

Schild-Poulter C,Matheos D,Novac O,Cui B,Giffin W,Ruiz MT,Price GB,Zannis-Hadjopoulos M,Haché RJ

MA5-12938 was used in EMSA to study the role of differential DNA binding of Ku antigen in determining its involvement in DNA replication

Sat Feb 01 00:00:00 UTC 2003

In vivo association of Ku with mammalian origins of DNA replication.

Molecular biology of the cell

Novac O,Matheos D,Araujo FD,Price GB,Zannis-Hadjopoulos M

MA5-12938 was used in immunoprecipitation to investigate the role of Ku protein in the DNA replication in mammalia

Thu Nov 01 00:00:00 UTC 2001

Ku entry into DNA inhibits inward DNA transactions in vitro.

The Journal of biological chemistry

Frit P,Li RY,Arzel D,Salles B,Calsou P

MA5-12938 was used in immunoprecipitation to study the effect of Ku entry into DNA on local DNA processes and the regulatory role of DNA-dependent protein kinase

Fri Nov 17 00:00:00 UTC 2000

The repressor which binds the -75 GATA motif of the GPB promoter contains Ku70 as the DNA binding subunit.

Nucleic acids research

Camara-Clayette V,Thomas D,Rahuel C,Barbey R,Cartron JP,Bertrand O

MA5-12938 was used in EMSA to study the role of the Ku70 protein in transcriptionally regulating the erythroid glycoprotein B promoter

Thu Apr 01 00:00:00 UTC 1999

Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes.

The EMBO journal

Shao RG,Cao CX,Zhang H,Kohn KW,Wold MS,Pommier Y

MA5-12938 was used in immunoprecipitation to study the role of replication protein A in the replication-mediated DNA damage caused by the topoisomerase inhibitor camptothecin

Mon Mar 01 00:00:00 UTC 1999

Physical interaction between epidermal growth factor receptor and DNA-dependent protein kinase in mammalian cells.

The Journal of biological chemistry

Bandyopadhyay D,Mandal M,Adam L,Mendelsohn J,Kumar R

MA5-12938 was used in western blot to study the biological functions of the epidermal growth factor receptor and DNA-dependent protein kinase interaction

Fri Jan 16 00:00:00 UTC 1998

生物信息学

蛋白别名: 5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 5'-dRP/AP lyase Ku70; 70 kDa subunit of Ku antigen; 86 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 70 kDa subunit; ATP-dependent DNA helicase II 80 kDa subunit; ATP-dependent DNA helicase II, 70 kDa subunit; CTC box binding factor 75 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC box-binding factor 85 kDa subunit; CTC75; CTC85; CTCBF; DNA repair protein XRCC5; DNA repair protein XRCC6; Ku autoantigen p70 subunit; Ku autoantigen protein p70 homolog; Ku autoantigen protein p86 homolog; Ku autoantigen, 70kDa; Ku autoantigen, 80kDa; Ku p70; Ku p80; Ku70; Ku80; Ku86; Ku86 autoantigen related protein 1; Lupus Ku autoantigen protein p70; Lupus Ku autoantigen protein p86; Nuclear factor IV; thyroid autoantigen 70 kDa; thyroid autoantigen 70kD (Ku antigen); thyroid autoantigen 70kDa (Ku antigen); Thyroid-lupus autoantigen; thyroid-lupus autoantigen p70; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining); X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining; Ku autoantigen, 80kDa); X-ray repair complementing defective repair in Chinese hamster cells 6; X-ray repair cross-complementing protein 5; X-ray repair cross-complementing protein 6

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基因别名: 70kDa; AI314015; CTC75; CTCBF; G22P1; G22P2; KARP-1; KARP1; KU70; KU80; Ku86; KUB2; ML8; NFIV; TLAA; XELAEV_18023498mg; XELAEV_18044412mg; XRCC5; xrcc5-a; xrcc5.L; XRCC6

查看更多收起

功能

DNA binding damaged DNA binding double-stranded DNA binding double-stranded telomeric DNA binding ATP-dependent DNA helicase activity protein binding ATP binding protein C-terminus binding ubiquitin protein ligase binding telomeric DNA binding transcription regulatory region DNA binding poly(A) RNA binding macromolecular complex binding 5'-deoxyribose-5-phosphate lyase activity nucleotide binding helicase activity hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity lyase activity DNA helicase

参与通路

telomere maintenance double-strand break repair double-strand break repair via nonhomologous end joining DNA recombination transcription, DNA-templated cell proliferation positive regulation of type I interferon production DNA duplex unwinding negative regulation of transcription, DNA-templated regulation of smooth muscle cell proliferation positive regulation of neurogenesis hematopoietic stem cell differentiation cellular response to gamma radiation cellular response to X-ray establishment of integrated proviral latency negative regulation of t-circle formation DNA ligation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter protein heterotetramerization double-strand break repair via classical nonhomologous end joining DNA repair regulation of transcription, DNA-templated cellular response to DNA damage stimulus cellular hyperosmotic salinity response metabolic process response to ionizing radiation V(D)J recombination

Ku70/Ku80 Monoclonal Antibody (162)

Ku70/Ku80 Antibody (MA5-12938) in ICC/IF

产品信息

MA5-12938

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

免疫沉淀 (IP)

染色质免疫沉淀实验 (ChIP)

凝胶阻滞分析 (GS)

阻断反应 (BLOCK)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

Ku70/Ku80 Monoclonal Antibody (162)

Ku70/Ku80 Antibody (MA5-12938) in ICC/IF

产品信息

MA5-12938

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

免疫沉淀 (IP)

染色质免疫沉淀 实验 (ChIP)

凝胶阻滞分析 (GS)

阻断反应 (BLOCK)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)