Invitrogen

Ku80 Monoclonal Antibody (5C5)

Ku80 Antibody in Immunocytochemistry (ICC/IF)

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Ku80 Antibody (MA5-15873) in ICC/IF

Immunofluorescence analysis of HeLa cells using XRCC5 monoclonal antibody (Product # MA5-15873) (Green). Red: actin filaments have been labeled with phalloidin.

Ku80 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Ku80 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with Ku80 siRNA and decrease in signal was observed in Western Blot using Ku80 Monoclonal Antibody (5C5) (Product # MA5-15873). {KD}

产品信息

MA5-15873

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看5篇已发表文章 5篇已发表文章

免疫组化（石蜡） (IHC (P))

1:200-1:1,000

免疫细胞化学 (ICC/IF)

1:200-1:1,000

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

1:200-1:400

酶联免疫吸附实验 (ELISA)

1:10,000

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

5C5

抗原

Purified recombinant fragment of human XRCC5 expressed in E. Coli.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

ascites

内含物

0.03% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_11156182

产品详细信息

MA5-15873 targets XRCC5 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human and mouse samples.

The MA5-15873 immunogen is purified recombinant fragment of human XRCC5 expressed in E. Coli. .

MA5-15873 detects XRCC5 which has a predicted molecular weight of approximately 86kDa.

靶标信息

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Photoreceptor laminin drives differentiation of human pluripotent stem cells to photoreceptor progenitors that partially restore retina function.

Molecular therapy : the journal of the American Society of Gene Therapy

Tay HG,Andre H,Chrysostomou V,Adusumalli S,Guo J,Ren X,Tan WS,Tor JE,Moreno-Moral A,Plastino F,Bartuma H,Cai Z,Tun SBB,Barathi VA,Siew Wei GT,Grenci G,Chong LY,Holmgren A,Kvanta A,Crowston JG,Petretto E,Tryggvason K

MA5-15873 was used in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot to investigate the role of a human recombinant retina-specific laminin isoform, LN523 in promoting the differentiation of human embryonic stem cells into photoreceptor progenitors.

Wed Mar 01 00:00:00 UTC 2023

Lysine-specific demethylase 2A enhances binding of various nuclear factors to CpG-rich genomic DNAs by action of its CXXC-PHD domain.

Scientific reports

Iuchi S,Paulo JA

MA5-15873 was used in Western Blotting to propose that CXXC-PHD promotes permissiveness for nuclear factors to interact, but involvement of the XRCC5/6 complex in the recruitment is undetermined.

Tue Apr 02 00:00:00 UTC 2019

CAPRI enables comparison of evolutionarily conserved RNA interacting regions.

Nature communications

Panhale A,Richter FM,Ramírez F,Shvedunova M,Manke T,Mittler G,Akhtar A

MA5-15873 was used in Western Blotting to produce a method to map RNA-binding domains by simultaneous identification of RNA interacting crosslinked peptides and peptides adjacent to such crosslinked sites.

Tue Jun 18 00:00:00 UTC 2019

Tyrosine kinase inhibitor-induced defects in DNA repair sensitize FLT3(ITD)-positive leukemia cells to PARP1 inhibitors.

Blood

Maifrede S,Nieborowska-Skorska M,Sullivan-Reed K,Dasgupta Y,Podszywalow-Bartnicka P,Le BV,Solecka M,Lian Z,Belyaeva EA,Nersesyan A,Machnicki MM,Toma M,Chatain N,Rydzanicz M,Zhao H,Jelinek J,Piwocka K,Sliwinski T,Stoklosa T,Ploski R,Fischer T,Sykes SM,Koschmieder S,Bullinger L,Valent P,Wasik MA,Huang J,Skorski T

MA5-15873 was used in Western Blotting to suggest FLT3(ITD) could be used as a precision medicine marker for identifying AML patients that may benefit from a therapeutic regimen combining FLT3 and PARP1i's.

Thu Jul 05 00:00:00 UTC 2018

Gene expression and mutation-guided synthetic lethality eradicates proliferating and quiescent leukemia cells.

The Journal of clinical investigation

Nieborowska-Skorska M,Sullivan K,Dasgupta Y,Podszywalow-Bartnicka P,Hoser G,Maifrede S,Martinez E,Di Marcantonio D,Bolton-Gillespie E,Cramer-Morales K,Lee J,Li M,Slupianek A,Gritsyuk D,Cerny-Reiterer S,Seferynska I,Stoklosa T,Bullinger L,Zhao H,Gorbunova V,Piwocka K,Valent P,Civin CI,Muschen M,Dick JE,Wang JC,Bhatia S,Bhatia R,Eppert K,Minden MD,Sykes SM,Skorski T

MA5-15873 was used in Western Blot to conclude that GEMA-guided targeting of PARP1 resulted in dual cellular synthetic lethality in quiescent and proliferating immature leukemia cells, and is thus a potential approach to eradicate leukemia stem and progenitor cells that are responsible for initiation and manifestation of the disease.

Thu Jun 01 00:00:00 UTC 2017

IGH/MYC Translocation Associates with BRCA2 Deficiency and Synthetic Lethality to PARP1 Inhibitors.

Molecular cancer research : MCR

Maifrede S,Martin K,Podszywalow-Bartnicka P,Sullivan-Reed K,Langer SK,Nejati R,Dasgupta Y,Hulse M,Gritsyuk D,Nieborowska-Skorska M,Lupey-Green LN,Zhao H,Piwocka K,Wasik MA,Tempera I,Skorski T

MA5-15873 was used in Western Blotting to demonstrate that untreated and cytarabine (AraC)-treated IGH/MYC-positive BL cells accumulate a high number of potentially lethal DNA double-strand breaks (DSBs) and display low levels of the BRCA2 tumor suppressor protein, which is a key element of homologous recombination (HR)-mediated DSB repair.

Tue Aug 01 00:00:00 UTC 2017

生物信息学

蛋白别名: 86 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; FLJ39089; HGNC:12833; Ku autoantigen protein p86 homolog; Ku autoantigen, 80kDa; Ku p80; Ku-80; Ku-86; Ku80; Ku86; Ku86 autoantigen related protein 1; Lupu; lupus; Lupus Ku autoantigen protein p86; Nuclear factor IV; OTTHUMP00000164061; OTTHUMP00000206791; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining); X-ray repair cross-complementing protein 5

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基因别名: AI314015; G22P2; KARP-1; KARP1; KU80; Ku86; KUB2; NFIV; XRCC5

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UniProt ID: (Human) P13010, (Mouse) P27641

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Entrez Gene ID: (Human) 7520, (Mouse) 22596

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功能