Invitrogen

LAP1 Monoclonal Antibody (RL13)

LAP1 Antibody in Immunocytochemistry (ICC/IF)

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LAP1 Antibody (MA1-074) in ICC/IF

Immunofluorescent analysis of LAP1 using anti-LAP1 monoclonal antibody (Product # MA1-074) shows staining in NS-1 Cells.

LAP1 Antibody in Immunohistochemistry (IHC)

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产品信息

MA1-074

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:200

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免疫组化（石蜡） (IHC (P))

1:20-1:50

免疫细胞化学 (ICC/IF)

1:100

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免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

RL13

抗原

Pore complex-lamina fraction isolated from rat liver nuclear envelopes.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2056886

产品详细信息

MA1-074 detects lamin associated polypeptide (LAP) 1A, 1B & 1C from rat, human, and mouse samples. This antibody does not cross-react with LAP 2.

MA1-074 has been successfully used in Western blot, immunofluorescence, immunohistochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects 3 distinct proteins of molecular weights ~65, ~56 and ~ 51 kDa representing LAP 1A, 1B, and 1C, respectively in rat liver nuclear envelope fractions. Immunofluorescence staining of LAP 1 in rat liver with MA1-074 results in exclusive labeling of the nuclear periphery and exhibits co-localization with lamin staining.

The MA1-074 immunogen is pore complex-lamina fraction isolated from rat liver nuclear envelopes.

靶标信息

Exopeptidase which selectively removes arginine and/or lysine residues from the N-terminus of several peptide substrates including Arg(0)-Leu-enkephalin, Arg(0)-Met-enkephalin and Arg(-1)-Lys(0)-somatostatin-14. Can hydrolyze leukotriene A4 (LTA-4) into leukotriene B4 (LTB-4).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Physical evidence on desmin-lamin B interaction.

Cytoskeleton (Hoboken, N.J.)

Kural-Mangıt E,Dinçer PR

MA1-074 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to show a physical interaction between desmin and lamin B via reciprocal co-immunoprecipitation from muscle tissue of wild type AB zebrafish (Danio rerio, Hamilton).

Fri Jan 01 00:00:00 UTC 2021

SUN1 interacts with nuclear lamin A and cytoplasmic nesprins to provide a physical connection between the nuclear lamina and the cytoskeleton.

Molecular and cellular biology

Haque F,Lloyd DJ,Smallwood DT,Dent CL,Shanahan CM,Fry AM,Trembath RC,Shackleton S

MA1-074 was used in western blot to study the role of SUN1 in providing a physical link between the nuclear lamina and the cytoskeleton

Mon May 01 00:00:00 UTC 2006

Lamin-binding fragment of LAP2 inhibits increase in nuclear volume during the cell cycle and progression into S phase.

The Journal of cell biology

Yang L,Guan T,Gerace L

MA1-074 was used in immunocytochemistry to investigate the functional properties of lamin-binding fragment of LAP2 during the cell cycle and progression into S phase

Mon Dec 01 00:00:00 UTC 1997

生物信息学

蛋白别名: Lamin-associated protein 1B; Lamina-associated polypeptide 1B; Lamina-associated polypeptide 1C; LAP1B; LAP1C; torsin A interacting protein 1; Torsin-1A-interacting protein 1

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基因别名: LAP1; LAP1B; Lap1c; TOR1AIP1

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UniProt ID: (Human) Q5JTV8, (Rat) Q5PQX1, (Mouse) Q921T2

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Entrez Gene ID: (Human) 26092, (Rat) 246314, (Mouse) 208263

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功能

ATPase activator activity protein binding lamin binding cytoskeletal protein binding ATPase binding

参与通路

positive regulation of ATPase activity protein localization to nucleus nuclear membrane organization