Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 9
LAT Antibody (PA5-78472) in ICC/IF
Immunofluorescence analysis of LAT was performed using Jurkat cells as positive model and Raji as negative. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with LAT Polyclonal Antibody (Product # PA5-78472) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # ... View More
产品信息
PA5-78472
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human LAT. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
1.41 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2736083
产品详细信息
Positive Control: Jurkat, Jurkat membrane extract
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: 36 kDa phospho-tyrosine adapter protein; 36 kDa phospho-tyrosine adaptor protein; 36 kDa phosphotyrosine adapter protein; linker for activation of T cells; linker for activation of T cells, transmembrane adaptor; Linker for activation of T-cells family member 1; linker protein; p36-38; pp36
基因别名: LAT; LAT1; pp36
UniProt ID: (Human) O43561, (Mouse) O54957
Entrez Gene ID: (Human) 27040, (Mouse) 16797
MAPK cascade
adaptive immune response
lymphocyte homeostasis
inflammatory response
immune response
integrin-mediated signaling pathway
Ras protein signal transduction
positive regulation of signal transduction
gene expression
calcium-mediated signaling
platelet activation
intracellular signal transduction
Fc-epsilon receptor signaling pathway
T cell activation
mast cell degranulation
positive regulation of GTPase activity
positive regulation of protein kinase activity
T cell receptor signaling pathway
regulation of T cell activation
immune system process
cellular defense response
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
homeostasis of number of cells
