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LATS1/LATS2 Antibody (PA5-115498) in ICC/IF
Immunocytochemistry analysis of LATS1/LATS2 in HeLa. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115498) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with Alexa Fluor 594 conjugated goat anti-rabbit IgG using a dilution of 1:600.
产品信息
PA5-115498
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human LATS1(Accession O95835), corresponding to amino acid residues C887-G937.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2900134
产品详细信息
Antibody detects endogenous levels of total LATS1/2.
靶标信息
The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: h-warts; Kinase phosphorylated during mitosis protein; Large tumor suppressor homolog 1; Large tumor suppressor homolog 2; large tumor supressor, homolog 1; LATS (large tumor suppressor, Drosophila) homolog 1; LATS (large tumor suppressor, Drosophila) homolog 2; LATS, large tumor suppressor, homolog 1; LATS, large tumor suppressor, homolog 2; LATS1; LATS2; serine/threonine kinase KPM; Serine/threonine-protein kinase kpm; Serine/threonine-protein kinase LATS1; Serine/threonine-protein kinase LATS2; WARTS protein kinase; Warts-like kinase
基因别名: 4932411G09Rik; AV277261; AW208599; AW228608; KPM; LATS1; LATS2; WARTS; wts
UniProt ID: (Human) O95835, (Human) Q9NRM7, (Mouse) Q8BYR2, (Mouse) Q7TSJ6
Entrez Gene ID: (Human) 9113, (Human) 26524, (Mouse) 16798, (Mouse) 50523
magnesium ion binding
protein serine/threonine kinase activity
protein binding
ATP binding
protein kinase binding
metal ion binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
G1/S transition of mitotic cell cycle
G2/M transition of mitotic cell cycle
sister chromatid segregation
inner cell mass cell fate commitment
inner cell mass cellular morphogenesis
protein phosphorylation
mitotic nuclear division
hormone-mediated signaling pathway
keratinocyte differentiation
regulation of actin filament polymerization
positive regulation of peptidyl-serine phosphorylation
hippo signaling
positive regulation of apoptotic process
regulation of protein complex assembly
negative regulation of cyclin-dependent protein serine/threonine kinase activity
regulation of organ growth
cytoplasmic sequestering of protein
cell division
negative regulation of canonical Wnt signaling pathway
peptidyl-serine phosphorylation
cellular protein localization
intracellular signal transduction
cell cycle
phosphorylation
