Invitrogen

LATS2 Recombinant Rabbit Monoclonal Antibody (17H14L2)

LATS2 Antibody in Immunocytochemistry (ICC/IF)

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LATS2 Antibody (703621) in ICC/IF

For immunofluorescence analysis, U-2 OS cells were fixed and permeabilized for detection of endogenous LATS2 using Anti-LATS2 Recombinant Rabbit Monoclonal Antibody (Product # 703621, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962) and cytoskeletal F-actin (red) staining using Rhodamine Phalloidin (Product # R415, 1:300) Panel a-d) shows ... View More

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LATS2 Antibody — Detection of subcellular localization of the target protein by cell treatment demonstrates antibody specificity. Immunofluorescence analysis of LATS2 using Anti-LATS2 Recombinant Rabbit Monoclonal Antibody (Product # 703621) showing staining in the cytoplasm of U-2 OS cells treated with Nocodozole in comparison to control cells. {TM}

产品信息

703621

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

17H14L2

抗原

Protein corresponding to human LATS2 [aa500-aa648]

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2762394

产品详细信息

This antibody is predicted to react with Monkey, Horse, Rat, Mouse.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

LATS2 is a serine/threonine protein kinase belonging to the LATS tumor suppressor family. LATS2 interacts with a negative regulator of p53 and function in a positive feedback loop with p53 that responds to cytoskeleton damage and this interaction provokes centrosome/mitotic apparatus dysfunction. LATS2 plays an essential role in the maintenance of mitotic fidelity and genomic integrity. LATS2 plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. It negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: h-warts; Kinase phosphorylated during mitosis protein; Large tumor suppressor homolog 2; LATS (large tumor suppressor, Drosophila) homolog 2; LATS, large tumor suppressor, homolog 2; LATS2; serine/threonine kinase KPM; Serine/threonine-protein kinase kpm; Serine/threonine-protein kinase LATS2; Warts-like kinase

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基因别名: KPM; LATS2

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UniProt ID: (Human) Q9NRM7

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Entrez Gene ID: (Human) 26524

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功能

protein serine/threonine kinase activity protein binding ATP binding metal ion binding non-receptor serine/threonine protein kinase

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