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LC3A (Cleaved Gly120) Antibody (PA5-35196) in ICC/IF
Immunocytochemistry analysis of LC3A (Cleaved Gly120) in HeLa cells. Samples were incubated with LC3A (Cleaved Gly120) polyclonal antibody (Product # PA5-35196) using a dilution of 0.0590277777777778 followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG at a dilution of 1:400 (green). Cells were 4% paraformaldehyde-fixed and 0.1% Triton X-100 permeabilized. Immunofluorescence image showing vesicles st... View More
产品信息
PA5-35196
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
KLH conjugated synthetic peptide between 89-120 amino acids from human Cleaved LC3A or LC3B
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A, Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2552506
产品详细信息
This antibody is predicted to react with bovine and rat based on sequence homology.
靶标信息
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/1B light chain 3 A; MAP1A/MAP1B LC3 A; MAP1A/MAP1B light chain 3 A; Microtubule-associated protein 1 light chain; Microtubule-associated protein 1 light chain 3 alpha; microtubule-associated proteins 1A/1B light chain 3; Microtubule-associated proteins 1A/1B light chain 3A
基因别名: 1010001H21Rik; 4922501H04Rik; ATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3; MAP1LC3A
UniProt ID: (Human) Q9H492, (Mouse) Q91VR7
Entrez Gene ID: (Human) 84557, (Mouse) 66734
