Invitrogen

LDHA Polyclonal Antibody

LDHA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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LDHA Antibody (PA5-26531) in IHC (P)

Immunohistochemistry analysis of LDHA in formalin-fixed and paraffin-embedded human skin. Samples were incubated with LDHA polyclonal antibody (Product # PA5-26531) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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产品信息

PA5-26531

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看4篇已发表文章 4篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:100

免疫细胞化学 (ICC/IF)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

1:10-1:50

产品规格

种属反应

Human

已发表种属

Fruit fly, Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 154-182 amino acids from the central region of human LDHA

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.34 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2544031

产品详细信息

This antibody is predicted to react with bovine, porcine, non-human primate and rat based on sequence homology.

靶标信息

Lactate dehydrogenase (LDH) is the enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+. In mammals, the enzyme is encoded by three genes: LDHA (M or muscle form), LDHB (H or heart form), and LDHC (X or testis form). The three human LDHs have 84-89% sequence similarities and 69-75% amino acid identities. There are five different LDH isoenzymes based on the proportion of M and H chains existing in the LDH tetrameric structure. The LDHA is hypoxia inducible and its expression is directly controlled by the transcriptional activity of the hypoxia inducible factor 1a (HIF1a). LDHB is also known to be upregulated in many cancers. Mutations in the LDHA gene can lead to Glycogen storage disease 11 (GSD11).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

eIF4EHP promotes Ldh mRNA translation in and fruit fly adaptation to hypoxia.

EMBO reports

Liang M,Hody C,Yammine V,Soin R,Sun Y,Lin X,Tian X,Meurs R,Perdrau C,Delacourt N,Oumalis M,Andris F,Conrard L,Kruys V,Gueydan C

PA5-26531 was used in Immunocytochemistry to show that eIF4EHP is necessary for Drosophila development under low oxygen concentrations and contributes to Drosophila mobility after hypoxic challenge.

Wed Jul 05 00:00:00 UTC 2023

eIF4EHP promotes Ldh mRNA translation in and fruit fly adaptation to hypoxia.

EMBO reports

Liang M,Hody C,Yammine V,Soin R,Sun Y,Lin X,Tian X,Meurs R,Perdrau C,Delacourt N,Oumalis M,Andris F,Conrard L,Kruys V,Gueydan C

PA5-26531 was used in Immunocytochemistry to show that eIF4EHP is necessary for Drosophila development under low oxygen concentrations and contributes to Drosophila mobility after hypoxic challenge.

Wed Jul 05 00:00:00 UTC 2023

Aging and memory are altered by genetically manipulating lactate dehydrogenase in the neurons or glia of flies.

Aging

Frame AK,Robinson JW,Mahmoudzadeh NH,Tennessen JM,Simon AF,Cumming RC

PA5-26531 was used in Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot to indicate that the direct alteration of lactate metabolism in either glia or neurons affects memory and survival but only in an age-dependent manner.

Tue Feb 28 00:00:00 UTC 2023

CircRNA circYY1 (hsa_circ_0101187) Modulates Cell Glycolysis and Malignancy Through Regulating YY1 Expression by Sponging miR-769-3p in Breast Cancer.

Cancer management and research

Zhang X,Li J,Feng Q

PA5-26531 was used in Western Blot to conclude that CircYY1 promoted glycolysis and tumor growth via increasing YY1 expression through sponging miR-769-3p in BC, offering a promising therapeutic target and prognostic biomarker for BC.

Wed Apr 20 00:00:00 UTC 2022

CircRNA circYY1 (hsa_circ_0101187) Modulates Cell Glycolysis and Malignancy Through Regulating YY1 Expression by Sponging miR-769-3p in Breast Cancer.

Cancer management and research

Zhang X,Li J,Feng Q

Wed Apr 20 00:00:00 UTC 2022

Lactate dehydrogenase expression modulates longevity and neurodegeneration in Drosophila melanogaster.

Aging

Long DM,Frame AK,Reardon PN,Cumming RC,Hendrix DA,Kretzschmar D,Giebultowicz JM

PA5-26531 was used in Western Blotting to show that Ldh transcript levels are elevated and cycle with diurnal rhythm in the heads of aged flies and this is associated with increased LDH protein, enzyme activity, and lactate concentrations.

Tue Jun 02 00:00:00 UTC 2020

Lactate dehydrogenase expression modulates longevity and neurodegeneration in Drosophila melanogaster.

Aging

Long DM,Frame AK,Reardon PN,Cumming RC,Hendrix DA,Kretzschmar D,Giebultowicz JM

Tue Jun 02 00:00:00 UTC 2020

生物信息学

蛋白别名: Cell proliferation-inducing gene 19 protein; epididymis secretory sperm binding protein Li 133P; L-lactate dehydrogenase; L-lactate dehydrogenase A chain; Lactate Dehydrogenase; lactate dehydrogenase M; Lactic Dehydrogenase; LDH; LDH muscle subunit; LDH-A; LDH-M; OTTHUMP00000165206; proliferation-inducing gene 19; Renal carcinoma antigen NY-REN-59

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基因别名: GSD11; HEL-S-133P; LDHA; LDHM; PIG19

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UniProt ID: (Human) P00338

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Entrez Gene ID: (Human) 3939

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功能

L-lactate dehydrogenase activity protein binding kinase binding identical protein binding NAD binding cadherin binding involved in cell-cell adhesion dehydrogenase

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