Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 4
LDHA Antibody (711782) in ICC/IF
For immunofluorescence analysis, PC3 cells were fixed and permeabilized for detection of endogenous LDHA using Anti- LDHA Recombinant Rabbit Superclonal™ Antibody (Product # 711782, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of LDHA protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938... View More
产品信息
711782
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
抗原
Peptides corresponding to human LDHA [1) aa211-aa229 2) aa2-aa19]
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2716911
产品详细信息
This antibody is predicted to react with Monkey, Cat, Pig
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
靶标信息
Lactate dehydrogenase (LDH) is the enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+. In mammals, the enzyme is encoded by three genes: LDHA (M or muscle form), LDHB (H or heart form), and LDHC (X or testis form). The three human LDHs have 84-89% sequence similarities and 69-75% amino acid identities. There are five different LDH isoenzymes based on the proportion of M and H chains existing in the LDH tetrameric structure. The LDHA is hypoxia inducible and its expression is directly controlled by the transcriptional activity of the hypoxia inducible factor 1a (HIF1a). LDHB is also known to be upregulated in many cancers. Mutations in the LDHA gene can lead to Glycogen storage disease 11 (GSD11).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: Cell proliferation-inducing gene 19 protein; epididymis secretory sperm binding protein Li 133P; L-lactate dehydrogenase; L-lactate dehydrogenase A chain; Lactate Dehydrogenase; lactate dehydrogenase M; Lactic Dehydrogenase; LDH; LDH muscle subunit; LDH-A; LDH-M; OTTHUMP00000165206; proliferation-inducing gene 19; Renal carcinoma antigen NY-REN-59
基因别名: GSD11; HEL-S-133P; LDHA; LDHM; PIG19
UniProt ID: (Human) P00338
Entrez Gene ID: (Human) 3939
response to hypoxia
lactate metabolic process
pyruvate metabolic process
glycolytic process
response to nutrient
response to glucose
NAD metabolic process
response to drug
response to hydrogen peroxide
positive regulation of apoptotic process
response to estrogen
post-embryonic organ development
response to cAMP
oxidation-reduction process
cell-cell adhesion
