Invitrogen

LDHB Polyclonal Antibody

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7 篇参考文献

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LDHB Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LDHB Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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LDHB Antibody (PA5-27505) in IHC (P)

LDHB Polyclonal Antibody detects LDHB protein at cytosol on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. LDHB Polyclonal Antibody (Product # PA5-27505) dilution: 1:200. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

LDHB Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LDHB Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LDHB Antibody in Western Blot (WB)

LDHB Antibody in Western Blot (WB)

LDHB Antibody in Western Blot (WB)

LDHB Antibody in Western Blot (WB)

LDHB Antibody in Western Blot, Immunohistochemistry (WB, IHC)

LDHB Antibody in Western Blot (WB)

LDHB Antibody in Immunomicroscopy (IM)

Product Image Gallery

LDHB Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — LDHB Polyclonal Antibody detects LDHB protein at cytosol on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. LDHB Polyclonal Antibody (Product # PA5-27505) dilution: 1:200. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

LDHB Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — LDHB Polyclonal Antibody detects LDH-B protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. LDH-B stained by LDHB Polyclonal Antibody (Product # PA5-27505) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

LDHB Antibody in Western Blot (WB) — Western blot was performed using Anti-LDHB Polyclonal Antibody (Product # PA5-27505) and a 35 kDa band corresponding to L-lactate dehydrogenase B chain was observed across MDA-MB-231, HeLa, Jurkat and Mouse Kidney. Whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), HeLa (Lane 2), Jurkat (Lane 3), Hep G2 (Lane 4), MCF7 (Lane 5) and Mouse Kidney (Lane 6) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:8000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

LDHB Antibody in Western Blot (WB) — Western blot analysis of LDHB was performed by separating 50 µg of various tissue extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a LDHB Polyclonal Antibody (Product # PA5-27505) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

LDHB Antibody in Western Blot (WB) — Western Blot using LDHB Polyclonal Antibody (Product # PA5-27505). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDHB Polyclonal Antibody (Product # PA5-27505) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

LDHB Antibody in Western Blot (WB) — Western Blot analysis of LDHB was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a LDHB Polyclonal Antibody (Product # PA5-27505) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.

LDHB Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Figure 1 Schematic diagram showing the generation of Ldhb -/- mouse, experimental design, and verification of Ldhb -/- model. ( A ) Schematic diagram illustrating the Cre-loxP and Flp-FRT system using pBS- Ldhb KO vectors. Ldhb -/- mice were generated through gene knockout at the exon 3 loci. ( B ) Schematic diagram of experimental design, showing the period of osmotin treatment in Ldhb -/- mice and behavior test. ( C , D ) The Western blot analysis showing the expression of LDHB in the cortex, hippocampus, kidney, and liver of Ldhb -/- and WT mice. beta-actin was used as a loading control. The bands were quantified using ImageJ software, and the differences are represented by histograms. ( E ) Confocal microscopic images showing the expression of LDHB in the cortex and hippocampus of Ldhb -/- , WT mice. ( F ) Immunohistochemistry staining for LDHB in the cortex and hippocampus of Ldhb -/- , WT mice.

LDHB Antibody in Western Blot (WB) — Figure 4 Effect of KDAC inhibition on enzyme activities in A549 cells under normoxia and hypoxia. ( A , B ) The ATP- dependent 6-phosphofructokinase (PFK1) ( A ) and lactate dehydrogenase (LDH) ( B ) enzymatic activities were measured after 24 h of incubation, and activities were normalized to intracellular protein content in each condition. A549 cells were treated with 1 muM of TSA, 20 mM of NAM, and both 1 muM TSA and 20 mM NAM for 24 h of incubation under normoxia and hypoxia. Cells incubated in medium without KDACIs served as control. Bars represent the means +- standard error of the mean of three independent experiments. The asterisks above bars indicate statistically significant differences compared to normoxic control cells. The asterisks above curly brackets indicate statistically significant differences between hypoxic and normoxic treatments and between hypoxic treatments and hypoxic control cells. Statistical significance was assessed by a two-tailed Student''s t -test. *, p <= 0.05; **, p <= 0.01; ***, p <= 0.001. ( C ) Western blot images of HIF-1alpha and selected metabolic enzymes identified by iTRAQ. A549 cells were treated with 1 muM of TSA, 20 mM of NAM and both 1 muM TSA and 20 mM NAM for 24 h of incubation under normoxia and hypoxia. Cells incubated in medium without KDACIs served as control. Immunoblotting of hypoxia-inducible factor-1alpha (HIF-1alpha), glucose-6-phosphate isomerase (GPI), phosphofructokinase-1 (PFK1), fructose-bisphosphate aldolase C (A

LDHB Antibody in Immunomicroscopy (IM) — Published figure using LDHB polyclonal antibody (Product # PA5-27505) in Immunomicroscopy

LDHB Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of L-lactate dehydrogenase B chain was observed in MDA-MB-231, HeLa and Jurkat in comparison to Hep G2 and MCF7 using Anti-LDHB Polyclonal Antibody (Product # PA5-27505) in Western Blot. {RE}

产品信息

PA5-27505

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看4篇已发表文章 4篇已发表文章

免疫组化 (IHC)

-

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

-

免疫细胞化学 (ICC/IF)

1:100-1:1,000

-

in situ PLA (PLA)

-

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免疫电镜技术 (IM)

-

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 118 and 334 of Human LDH-B

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.32 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2544981

产品详细信息

Recommended positive controls: 293T, U87-MG, SK-N-SH, IMR32, SK-N-AS, mouse brain, rat brain.

Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (81%), Dog (100%), Pig (96%), Rabbit (100%), Chicken (87%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This gene encodes an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on the X chromosome and on chromosome. Multiple alternatively spliced variants, encoding the same protein, have been identified.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Glycolysis drives STING signaling to facilitate dendritic cell antitumor function.

The Journal of clinical investigation

Hu Z,Yu X,Ding R,Liu B,Gu C,Pan XW,Han Q,Zhang Y,Wan J,Cui XG,Sun J,Zou Q

PA5-27505 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to provide mechanistic insight into how the crosstalk of glycolytic metabolism and STING signaling enhances DC antitumor activity and can be harnessed to improve cancer therapies.

Mon Apr 03 00:00:00 UTC 2023

LDHB Deficiency Promotes Mitochondrial Dysfunction Mediated Oxidative Stress and Neurodegeneration in Adult Mouse Brain.

Antioxidants (Basel, Switzerland)

Park JS,Saeed K,Jo MH,Kim MW,Lee HJ,Park CB,Lee G,Kim MO

PA5-27505 was used in Western Blotting and Immunohistochemistry to test the hypothesis that LDHB deficiency plays an important role in oxidative stress-mediated neuroinflammation and neurodegeneration.

Fri Jan 28 00:00:00 UTC 2022

LDHB Deficiency Promotes Mitochondrial Dysfunction Mediated Oxidative Stress and Neurodegeneration in Adult Mouse Brain.

Antioxidants (Basel, Switzerland)

Park JS,Saeed K,Jo MH,Kim MW,Lee HJ,Park CB,Lee G,Kim MO

PA5-27505 was used in Western Blotting and Immunohistochemistry to test the hypothesis that LDHB deficiency plays an important role in oxidative stress-mediated neuroinflammation and neurodegeneration.

Fri Jan 28 00:00:00 UTC 2022

LDHB Deficiency Promotes Mitochondrial Dysfunction Mediated Oxidative Stress and Neurodegeneration in Adult Mouse Brain.

Antioxidants (Basel, Switzerland)

Park JS,Saeed K,Jo MH,Kim MW,Lee HJ,Park CB,Lee G,Kim MO

PA5-27505 was used in Western Blotting and Immunohistochemistry to test the hypothesis that LDHB deficiency plays an important role in oxidative stress-mediated neuroinflammation and neurodegeneration.

Fri Jan 28 00:00:00 UTC 2022

LDHB Deficiency Promotes Mitochondrial Dysfunction Mediated Oxidative Stress and Neurodegeneration in Adult Mouse Brain.

Antioxidants (Basel, Switzerland)

Park JS,Saeed K,Jo MH,Kim MW,Lee HJ,Park CB,Lee G,Kim MO

PA5-27505 was used in Western Blotting and Immunohistochemistry to test the hypothesis that LDHB deficiency plays an important role in oxidative stress-mediated neuroinflammation and neurodegeneration.

Fri Jan 28 00:00:00 UTC 2022

LDHB Deficiency Promotes Mitochondrial Dysfunction Mediated Oxidative Stress and Neurodegeneration in Adult Mouse Brain.

Antioxidants (Basel, Switzerland)

Park JS,Saeed K,Jo MH,Kim MW,Lee HJ,Park CB,Lee G,Kim MO

PA5-27505 was used in Western Blotting and Immunohistochemistry to test the hypothesis that LDHB deficiency plays an important role in oxidative stress-mediated neuroinflammation and neurodegeneration.

Fri Jan 28 00:00:00 UTC 2022

LDHB Deficiency Promotes Mitochondrial Dysfunction Mediated Oxidative Stress and Neurodegeneration in Adult Mouse Brain.

Antioxidants (Basel, Switzerland)

Park JS,Saeed K,Jo MH,Kim MW,Lee HJ,Park CB,Lee G,Kim MO

PA5-27505 was used in Western Blotting and Immunohistochemistry to test the hypothesis that LDHB deficiency plays an important role in oxidative stress-mediated neuroinflammation and neurodegeneration.

Fri Jan 28 00:00:00 UTC 2022

R-2-hydroxyglutarate attenuates aerobic glycolysis in leukemia by targeting the FTO/m⁶A/PFKP/LDHB axis.

Molecular cell

Qing Y,Dong L,Gao L,Li C,Li Y,Han L,Prince E,Tan B,Deng X,Wetzel C,Shen C,Gao M,Chen Z,Li W,Zhang B,Braas D,Ten Hoeve J,Sanchez GJ,Chen H,Chan LN,Chen CW,Ann D,Jiang L,Müschen M,Marcucci G,Plas DR,Li Z,Su R,Chen J

PA5-27505 was used in Western Blot to investigate the effect of R-2-hydroxyglutarate on cancer metabolism.

Thu Mar 04 00:00:00 UTC 2021

Quantitative Proteomic Approach Reveals Altered Metabolic Pathways in Response to the Inhibition of Lysine Deacetylases in A549 Cells under Normoxia and Hypoxia.

International journal of molecular sciences

Martín-Bernabé A,Tarragó-Celada J,Cunin V,Michelland S,Cortés R,Poignant J,Boyault C,Rachidi W,Bourgoin-Voillard S,Cascante M,Seve M

Published figure using LDHB polyclonal antibody (Product # PA5-27505) in Western Blot

Thu Mar 25 00:00:00 UTC 2021

Branched-chain ketoacids secreted by glioblastoma cells via MCT1 modulate macrophage phenotype.

EMBO reports

Silva LS,Poschet G,Nonnenmacher Y,Becker HM,Sapcariu S,Gaupel AC,Schlotter M,Wu Y,Kneisel N,Seiffert M,Hell R,Hiller K,Lichter P,Radlwimmer B

PA5-27505 was used in Proximity Ligation Assay to demonstrate that tumor-excreted BCKAs can be taken up and re-aminated to BCAAs by tumor-associated macrophages.

Fri Dec 01 00:00:00 UTC 2017

Lactate metabolism is associated with mammalian mitochondria.

Nature chemical biology

Chen YJ,Mahieu NG,Huang X,Singh M,Crawford PA,Johnson SL,Gross RW,Schaefer J,Patti GJ

PA527505 was used to determine if cytosolic lactate is metabolized by the mitochondria of fermenting mammalian cells

Tue Nov 01 00:00:00 UTC 2016

生物信息学

蛋白别名: epididymis secretory protein Li 281; L-lactate dehydrogenase B chain; lactate dehydrogenase H chain; Lactate dehydrogenease B; LDH heart subunit; LDH-B; LDH-H; LDHH; Renal carcinoma antigen NY-REN-46; testicular secretory protein Li 25

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基因别名: AI790582; H-Ldh; HEL-S-281; Ldh-2; LDH-B; LDH-H; Ldh2; LDHB; LDHBD; TRG-5

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UniProt ID: (Human) P07195, (Rat) P42123, (Mouse) P16125

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Entrez Gene ID: (Human) 3945, (Rat) 24534, (Mouse) 16832

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L-lactate dehydrogenase activity protein binding kinase binding identical protein binding NAD binding lactate dehydrogenase activity dehydrogenase

carbohydrate metabolic process lactate metabolic process pyruvate metabolic process NAD metabolic process oxidation-reduction process cellular carbohydrate metabolic process