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LDHD Antibody (MA5-36153) in ICC/IF
Immunocytochemical analysis of LDHD in MCF-7 cells using a LDHD monoclonal antibody (Product #MA5-36153). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI.
Product Details
MA5-36153
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE53-15
Immunogen
Recombinant protein within Human LDHD aa 368-507
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2890465
Target Information
LDHD belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. The similar protein in yeast has both D-lactate and D-glycerate dehydrogenase activities. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: D-lactate dehydrogenase; DLD; LDHD; Probable D-lactate dehydrogenase, mitochondrial
Gene Aliases: 4733401P21Rik; Ac2-202; D8Bwg1320e; DLD; LDHD
UniProt ID: (Human) Q86WU2, (Mouse) Q7TNG8
Entrez Gene ID: (Human) 197257, (Rat) 307858, (Mouse) 52815
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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