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LEPREL1 Antibody (PA5-96918) in WB
Western blot analysis of LEPREL1 in extracts of NCI-H460 cells. Samples were incubated with LEPREL1 Polyclonal antibody (Product # PA5-96918) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
产品信息
PA5-96918
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 400-700 of human LEPREL1 (NP_0606622)
偶联物
形式
Liquid
浓度
0.47 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2808720
产品详细信息
Immunogen sequence: IRYGGRQDEN RVPSGVNVEG AEVHGFSMGK KLSPKIDRDL REGGPLLYEN ITFVYNSEQL NGTQRVLLDN VLSEEQCREL HSVASGIMLV GDGYRGKTSP HTPNEKFEGA TVLKALKSGY EGRVPLKSAR LFYDISEKAR RIVESYFMLN STLYFSYTHM VCRTALSGQQ DRRNDLSHPI HADNCLLDPE ANECWKEPPA YTFRDYSALL YMNDDFEGGE FIFTEMDAKT VTASIKPKCG RMISFSSGGE NPHGVKAVTK GKRCAVALWF TLDPLYRELE RIQADEVIAI LDQEQQGKHE L; Positive Samples: NCI-H460; Cellular Location: Endoplasmic reticulum, Golgi apparatus
靶标信息
This gene encodes a member of the prolyl 3-hydroxylase subfamily of 2-oxo-glutarate-dependent dioxygenases. These enzymes play a critical role in collagen chain assembly, stability and cross-linking by catalyzing post-translational 3-hydroxylation of proline residues. Mutations in this gene are associated with nonsyndromic severe myopia with cataract and vitreoretinal degeneration, and downregulation of this gene may play a role in breast cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: leprecan-like 1; Leprecan-like protein 1; myxoid liposarcoma associated protein 4; Myxoid liposarcoma-associated protein 4; procollagen-proline 3-dioxygenase 2; Prolyl 3-hydroxylase 2; prolyl 3-hydroxylase 3
基因别名: 4832416N06; AW553532; LEPREL1; MCVD; MLAT4; P3H2
UniProt ID: (Human) Q8IVL5, (Mouse) Q8CG71, (Rat) Q4KLM6
Entrez Gene ID: (Human) 55214, (Mouse) 210530, (Rat) 288016
iron ion binding
procollagen-proline 3-dioxygenase activity
L-ascorbic acid binding
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
metal ion binding
dioxygenase activity
extracellular matrix glycoprotein
