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LIF Antibody (500-P39-1MG) in WB
Western Blot: To detect Human LIF by Western Blot analysis LIF Polyclonal Antibody (Product # 500-P39-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human LIF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P39-1MG
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Not Applicable
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Human LIF
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2930510
产品详细信息
AA Sequence of recombinant protein: SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human LIF. Anti-Human LIF-specific antibody was purified by affinity chromatography employing an immobilized Human LIF matrix.
Sandwich ELISA: To detect Human LIF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human LIF (500-P39Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human LIF.
Western Blot: To detect Human LIF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human LIF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
靶标信息
Leukemia inhibitory factor (LIF) is a 20 kDa protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF can be up-regulated by pro-inflammatory cytokines such as TNFα and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal.Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: cholinergic differentiation factor; D factor; differentiation inhibitory activity; differentiation-inducing factor; Differentiation-stimulating factor; hepatocyte-stimulating factor III; human interleukin in DA cells; Leukemia inhibitory factor; LIF; Melanoma-derived LPL inhibitor; MLPLI
基因别名: CDF; DIA; HILDA; LIF; MLPLI
UniProt ID: (Human) P15018
Entrez Gene ID: (Human) 3976
blood vessel remodeling
immune response
multicellular organism development
embryo implantation
positive regulation of cell proliferation
negative regulation of cell proliferation
positive regulation of neuron projection development
negative regulation of angiogenesis
stem cell population maintenance
organ regeneration
positive regulation of peptidyl-serine phosphorylation
positive regulation of peptidyl-serine phosphorylation of STAT protein
tyrosine phosphorylation of Stat3 protein
positive regulation of tyrosine phosphorylation of Stat1 protein
positive regulation of tyrosine phosphorylation of Stat3 protein
positive regulation of MAPK cascade
positive regulation of macrophage differentiation
negative regulation of meiotic nuclear division
positive regulation of transcription from RNA polymerase II promoter
decidualization
negative regulation of hormone secretion
lung alveolus development
muscle organ morphogenesis
neuron development
astrocyte differentiation
positive regulation of astrocyte differentiation
leukemia inhibitory factor signaling pathway
stem cell differentiation
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of corticotropin secretion
retina development in camera-type eye
transdifferentiation
lung vasculature development
lung lobe morphogenesis
trophoblast giant cell differentiation
spongiotrophoblast differentiation
negative regulation of ERK1 and ERK2 cascade
positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
regulation of metanephric nephron tubule epithelial cell differentiation
positive regulation of protein localization to nucleus
positive regulation of histone H3-K27 acetylation
regulation of RNA polymerase II regulatory region sequence-specific DNA binding
