Invitrogen

LIF Polyclonal Antibody

LIF Antibody in Immunocytochemistry (ICC/IF)

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LIF Antibody (PA5-79601) in ICC/IF

Immunofluorescence analysis of LIF was performed using 80% confluent log phase MDA-MB-231 and T-47D, control and PTI treated, cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with LIF Polyclonal Antibody (Product # PA5-79601) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa F... View More

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LIF Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-LIF Polyclonal Antibody (Product # PA5-79601), shows cytoplasmic accumulation of LIF in MDA-MB-231 cells upon PTI treatment but not in T-47D treated with the same. MDA-MB-231 is reported as high expressing for LIF in comparison to T-47D for tumorigenesis and metastasis [doi: 10.1158/1535-7163.MCT-18-1258; 10.18632/oncotarget.1772; 10.18632/oncotarget.6756] {RE}

产品信息

PA5-79601

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence at the C-terminus of mouse LIF (175-194aa DKEAFQRKKLGCQLLGTYKQ).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide, 0.05mg thimerosal

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2746716

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

Leukemia inhibitory factor (LIF) is a 20 kDa protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF can be up-regulated by pro-inflammatory cytokines such as TNFα and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal.Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: cholinergic neuronal differentiation factor; D factor; Differentiation-stimulating factor; Leukemia inhibitory factor; leukemia inhibitory factor (cholinergic differentiation factor); LIF; myeloid leukaemia inhibitory factor

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基因别名: Lif

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UniProt ID: (Mouse) P09056

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Entrez Gene ID: (Mouse) 16878, (Rat) 60584

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功能

transcription factor activity, RNA polymerase II transcription factor recruiting receptor binding cytokine activity leukemia inhibitory factor receptor binding growth factor activity cytokine intercellular signal molecule

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