Invitrogen

LIM1 Polyclonal Antibody

查看其他25个LIM1抗体

抗体检测数据 (3)

LIM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LIM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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LIM1 Antibody (PA5-106827) in IHC (P)

Immunohistochemistry analysis of paraffin-embedded LIM1 in human heart tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with LIM1 polyclonal antibody (Product # PA5-106827) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

LIM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LIM1 Antibody in Western Blot (WB)

LIM1 Antibody in Western Blot (WB)

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LIM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded LIM1 in human heart tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with LIM1 polyclonal antibody (Product # PA5-106827) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

LIM1 Antibody in Western Blot (WB) — Western blot analysis of LIM1 in HUVEC/HeLa cells. Samples were incubated with LIM1 polyclonal antibody (Product # PA5-106827).

LIM1 Antibody in Western Blot (WB) — Western blot analysis of LIM1 in HeLa cell lysate (left lane: treated with blocking peptide). Samples were incubated with LIM1 polyclonal antibody (Product # PA5-106827).

产品信息

PA5-106827

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human LHX1(Accession P48742), corresponding to amino acid residues P143-E193.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2854492

产品详细信息

Antibody detects endogenous levels of total LHX1.

靶标信息

This gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator and be involved in control of differentiation and development of neural and lymphoid cells. A similar protein in mice is an essential regulator of the vertebrate head organizer.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: hLim-1; Homeobox protein Lim-1; LHX-1; LIM homeo box protein 1; LIM homeobox protein 1; LIM/homeobox protein Lhx1; MGC126723; MGC138141; Rlim

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基因别名: LHX1; LIM-1; LIM1

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UniProt ID: (Human) P48742, (Mouse) P63006, (Rat) P63007

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Entrez Gene ID: (Human) 3975, (Mouse) 16869, (Rat) 257634

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transcription factor activity, sequence-specific DNA binding transcription corepressor activity zinc ion binding sequence-specific DNA binding DNA binding protein binding metal ion binding helix-turn-helix transcription factor homeodomain transcription factor DNA-binding transcription factor gene-specific transcriptional regulator

urogenital system development ureteric bud development branching involved in ureteric bud morphogenesis gastrulation with mouth forming second ectoderm formation endoderm formation kidney development transcription from RNA polymerase II promoter cell-cell signaling pattern specification process nervous system development motor neuron axon guidance anatomical structure morphogenesis post-embryonic development embryonic pattern specification organ morphogenesis anterior/posterior axis specification anterior/posterior pattern specification dorsal/ventral pattern formation regulation of gene expression retina layer formation ventral spinal cord development spinal cord association neuron differentiation telencephalon development cerebellum development cerebellar Purkinje cell differentiation forebrain regionalization cerebellar Purkinje cell-granule cell precursor cell signaling involved in regulation of granule cell precursor cell proliferation somite rostral/caudal axis specification metanephric part of ureteric bud development oviduct epithelium development uterine epithelium development nephric duct elongation horizontal cell localization positive regulation of embryonic development cellular response to fibroblast growth factor stimulus negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated anatomical structure formation involved in morphogenesis embryonic viscerocranium morphogenesis pronephros development embryonic retina morphogenesis in camera-type eye uterus development oviduct development cervix development vagina development head development epithelium development paramesonephric duct development comma-shaped body morphogenesis S-shaped body morphogenesis renal vesicle morphogenesis mesonephric duct development nephric duct morphogenesis metanephric glomerulus development metanephric comma-shaped body morphogenesis metanephric renal vesicle morphogenesis metanephric S-shaped body morphogenesis primitive streak formation positive regulation of branching involved in ureteric bud morphogenesis lateral motor column neuron migration positive regulation of gastrulation positive regulation of anterior head development positive regulation of nephron tubule epithelial cell differentiation transcription, DNA-templated regulation of transcription, DNA-templated multicellular organism development cell differentiation renal system development mesonephric tubule development