Invitrogen

LLGL1 Polyclonal Antibody

LLGL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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LLGL1 Antibody (PA5-54637) in IHC (P)

Immunohistochemical analysis of LLGL1 in human stomach using LLGL1 Polyclonal Antibody (Product # PA5-54637) shows strong cytoplasmic and membranous positivity in glandular cells.

LLGL1 Antibody in Immunohistochemistry (IHC)

LLGL1 Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

LLGL1 Antibody — Figure 6. DLD1 cells deficient for Llgl1/2 exhibit only mild polarity defects. (a) Western blotting of total cell lysates of DLDcas9 cells (DLD), their Llgl2-deficient clone (Llgl2-KO), and the clone of the latter clone obtained after Llgl1 knockout (Llgl1/2-KO). The lysates were probed with antibodies indicated at the bottom. Note that the anti-Lgl1 antibody used in our study (ab18302) cross-reacts with Llgl2. (b) Phase contrast of the Llgl2- and Llgl1/2-deficient colonies (Llgl2-KO and Llgl1/2-KO). Arrows indicate the sites of clear multilayered organization. (c and d) Projections of all x-y optical slices of Llgl2- and Llgl1/2-deficient cells stained for E-cad (green) and CGN (red). The optical z-cross-sections of these images at the same magnification are shown at the bottom. Bars, 10 um. (e and f) The confluent 3-d-old cultures of Llgl2-KO (e) and Llgl1/2-KO (f) cells were stained with anti-cingulin antibody (cingulin, red) and with antibodies against polarity proteins (green): hScrib (Scrib), Pals1, Par6B (Par6), and aPKCzeta (aPKC). Bars, 10 um. Separate and merged Z stacks for each antibody staining at the same magnification are provided in the right panel. {KO}

产品信息

PA5-54637

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

免疫组化（石蜡） (IHC (P))

1:200-1:500

免疫细胞化学 (ICC/IF)

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产品规格

种属反应

Human

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human LLGL1. Recombinant protein control fragment (Product #RP-93255).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.4 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2643396

产品详细信息

Immunogen sequence: FERFSLSARN ITEPLCSLDI NWPRDATQAS YRIRESPKLS QANGTPSILL APQSLDGSPD PAHSMGPDTP EPPEAALSPM SIDSATSADT TLDTTGDVTV EDVKDFLGSS EESEKNLRNL AEDE

Highest antigen sequence identity to the following orthologs: Mouse - 76%, Rat - 72%.

靶标信息

LLGL1 is a protein that is similar to a tumor suppressor in Drosophila. The protein is part of a cytoskeletal network and is associated with nonmuscle myosin II heavy chain and a kinase that specifically phosphorylates this protein at serine residues. The gene for LLGL1 is located within the Smith-Magenis syndrome region on chromosome 17.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Scribble, Erbin, and Lano redundantly regulate epithelial polarity and apical adhesion complex.

The Journal of cell biology

Choi J,Troyanovsky RB,Indra I,Mitchell BJ,Troyanovsky SM

Published figure using LLGL1 polyclonal antibody (Product # PA5-54637) in Immunocytochemistry

Mon Jul 01 00:00:00 UTC 2019

生物信息学

蛋白别名: DLG4; Hugl protein; Hugl-1; Human homolog to the D-lgl gene protein; lethal giant larvae homolog 1; lethal giant larvae homolog 1, scribble cell polarity complex component; Lethal(2) giant larvae protein homolog 1; LLGL

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基因别名: DLG4; HUGL; HUGL-1; HUGL1; Lgl1; LLGL; LLGL1; Mgl1

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UniProt ID: (Human) Q15334

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Entrez Gene ID: (Human) 3996

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功能

GTPase activator activity structural molecule activity protein binding Rab GTPase binding protein kinase binding syntaxin binding membrane trafficking regulatory protein

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