Invitrogen

LOX Polyclonal Antibody

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5 篇参考文献

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LOX Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LOX Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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LOX Antibody (PA1-16953) in IHC (P)

Immunohistochemical analysis of LOX in mouse stomach. Samples were incubated in LOX polyclonal antibody (Product # PA1-16953).

LOX Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LOX Antibody in Western Blot (WB)

LOX Antibody in Western Blot (WB)

LOX Antibody in Western Blot (WB)

LOX Antibody in Western Blot (WB)

LOX Antibody in Western Blot (WB)

LOX Antibody in Western Blot (WB)

LOX Antibody in Western Blot (WB)

LOX Antibody in Western Blot (WB)

LOX Antibody in Western Blot (WB)

LOX Antibody in Western Blot (WB)

LOX Antibody in Western Blot (WB)

LOX Antibody in Western Blot (WB)

Product Image Gallery

LOX Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of LOX in mouse stomach. Samples were incubated in LOX polyclonal antibody (Product # PA1-16953).

LOX Antibody in Western Blot (WB) — Western blot was performed using Anti-LOX Rabbit Polyclonal Antibody (Product # PA1-16953) and 47 kDa (canonical) and 60 kDa (glycosylated) bands corresponding to isoforms of LOX were observed in tissues tested except Mouse Spleen which is reported to be negative. Tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Mouse Kidney (Lane 2) and Mouse Spleen (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (2 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

LOX Antibody in Western Blot (WB) — Western blot analysis of LOX in 1.0 mg/mL HeLa cell lysate. Samples were incubated in LOX polyclonal antibody (Product # PA1-16953). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

LOX Antibody in Western Blot (WB) — Western blot analysis of LOX in human kidney. Sample was incubated in LOX polyclonal antibody (Product # PA1-16953).

LOX Antibody in Western Blot (WB) — 7 FIGURE The time course of LOXL2 mRNA and protein levels in the lungs following neonatal hypoxia exposure is distinct from the changes seen in isolated pulmonary arteries. (a) Loxl2 and (b) Lox gene expression in the lung of hypoxic neonates and normoxic littermate controls (fold change vs. P11 normoxia; 18S used as housekeeping gene; n = 6 per group; 3 male and 3 female; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-way ANOVA with Bonferroni post hoc analysis). (c) Representative Western blots of LOXL2 and Pro-LOX levels in the lung; active-LOX band was not detected. Densitometry analysis of (d) LOXL2 and (e) LOX protein in the ECM of lungs. n = 4-6 for Western blots, equal numbers of males and females; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-way ANOVA with Bonferroni post hoc analysis. Nx, normoxia, Hx, neonatal hypoxia from P1-P11.

LOX Antibody in Western Blot (WB) — 5 FIGURE LOXL2 mRNA and protein levels decrease rapidly in the heart after neonatal hypoxia exposure is terminated. (a) Loxl2 and (b) Lox gene expression in the heart with neonatal hypoxia exposure (Hx; fold change vs. normoxia (Nx) P11 hearts with 18S as housekeeping gene; 3 males, 3 females in each group). (c) Representative Western blots of LOXL2, Pro-LOX, and active-LOX protein levels in the ECM fraction of the heart. Densitometry analysis of (d) LOXL2, (e) Pro-LOX, and (f) Active LOX. For Western blots, n = 4-6; equal numbers of males and females; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-way ANOVA with Bonferroni post hoc analysis. Nx, normoxia, Hx, neonatal hypoxia from P1-P11.

LOX Antibody in Western Blot (WB) — 6 FIGURE Vascular stiffening and LOXL2 expression of the pulmonary artery after neonatal hypoxia exposure. (a) Tensile testing of pulmonary arteries of 60 day old mice exposed to postnatal hypoxia until day 11 compared with their normoxic littermate controls ( n = 6, 3 males and 3 females; p < 0.0001). (b) Representative Western blots of LOXL2, Pro-LOX, and active-LOX protein levels. Densitometry analysis of (c) LOXL2, (d) Pro- LOX, and (e) Active LOX. n = 4-6 for Western blots; equal numbers of males and females. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-way ANOVA with Bonferroni post hoc analysis. Nx, normoxia, Hx, neonatal hypoxia from P1-P11.

LOX Antibody in Western Blot (WB) — 4 FIGURE Aortic LOXL2 expression is elevated in adulthood after neonatal hypoxia exposure. (a) Representative Western blots for LOXL2, Pro-LOX, and active-LOX protein expression in the aortic ECM. Densitometry analysis of LOXL2 (b; 3 males, 2 females), Pro-LOX (c; 2 males, 2 females), and active (processed; 2 males, 2 females) LOX (d). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, by 2-way ANOVA with Bonferroni post hoc analysis. Nx = normoxia, Hx = neonatal hypoxia from P1-P11.

LOX Antibody in Western Blot (WB) — Figure 6 Expression level of protein of COX-2 and 5-LOX through western blot and levels of gene contributor through quantitative RT-PCR. Immunoblotting of respective individual group [I-Control (Normal saline, 3 ml/kg, p.o.), II- Toxic control (MNU 47 mg/kg, i.v.), III- Zaltoprofen (10 mg/kg, p.o. + MNU 47 mg/kg, i.v.), IV- Zileuton (10 mg/kg, p.o. + MNU 47 mg/kg, i.v.) and V- Zaltoprofen + Zileuton (5 + 5 mg/kg, p.o. + MNU 47 mg/kg, i.v.)] for COX-2 and 5-LOX. Excised mammary gland tissue sample lysed in trizol for RNA extraction and analyzed for the mRNA expression of COX-2 and 5-LOX by qRT-PCR. beta-actin was used as loading control. Each experiment was performed in triplicate. Values are presented as Mean +- SD. Comparisons were made by the one-way ANOVA followed by Bonferroni multiple test. All groups were compared to the MNU treated group ( ** p < 0.01, *** p < 0.001).

LOX Antibody in Western Blot (WB) — Published figure using LOX polyclonal antibody (Product # PA1-16953) in Western Blot

LOX Antibody in Western Blot (WB) — Published figure using LOX polyclonal antibody (Product # PA1-16953) in Western Blot

LOX Antibody in Western Blot (WB) — Published figure using LOX polyclonal antibody (Product # PA1-16953) in Western Blot

LOX Antibody in Western Blot (WB) — Published figure using LOX polyclonal antibody (Product # PA1-16953) in Western Blot

LOX Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues tested owing to their inherent genetic constitution. Relative expression of LOX was observed in Mouse Heart (Lane 1) and Mouse Kidney (Lane 2) that express LOX protein, in comparison to, Mouse Spleen that doesnt express LOX protein using Anti-LOX Rabbit Polyclonal Antibody (Product # PA1-16953) in Western Blot. {RE}

产品信息

PA1-16953

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5-4 µg/mL

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免疫组化（石蜡） (IHC (P))

1:100-1:250

-

免疫细胞化学 (ICC/IF)

1:100

-

产品规格

种属反应

Human, Mouse, Pig, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide between amino acids 200-300 of the human LOX protein.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_794775

产品详细信息

PA1-16953 is specific for the ~58 kDa LOX protein.

This is a highly glycosylated protein and therefore, other bands may be seen at ~32 kDa (non-glycosylated form) 50 kDa and 58 kDa (glycosylated forms).

Suggested positive control: human and mouse kidney lysate, antigen standard for LOX (transient overexpression lysate), human kidney protein, mouse kidney protein.

靶标信息

Lysyl oxidase (LOX), a copper-containing amine oxidase, belongs to a heterogeneous family of enzymes that oxidize primary amine substrates to reactive aldehydes. It plays a vital role in the formation and repair of the extra cellular matrix. As well, LOX is a multifunctional enzyme having diverse biological functions such as developmental regulation, tumor suppression, cell motility and cellular senescence. The secreted form of LOX is responsible for the invasive properties of hypoxic human cancer cells. Thus, it is essential for hypoxia-induced metastasis and is a good therapeutic target for preventing and treating metastases.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Neonatal exposure to hypoxia induces early arterial stiffening via activation of lysyl oxidases.

Physiological reports

Steppan J,Nandakumar K,Wang H,Jang R,Smith L,Kang S,Savage W,Bauer M,Choi R,Brady T,Wodu BP,Scafidi S,Scafidi J,Santhanam L

Published figure using LOX polyclonal antibody (Product # PA1-16953) in Western Blot

Sat Apr 01 00:00:00 UTC 2023

Neonatal exposure to hypoxia induces early arterial stiffening via activation of lysyl oxidases.

Physiological reports

Steppan J,Nandakumar K,Wang H,Jang R,Smith L,Kang S,Savage W,Bauer M,Choi R,Brady T,Wodu BP,Scafidi S,Scafidi J,Santhanam L

Published figure using LOX polyclonal antibody (Product # PA1-16953) in Western Blot

Sat Apr 01 00:00:00 UTC 2023

DuCLOX-2/5 Inhibition Attenuates Inflammatory Response and Induces Mitochondrial Apoptosis for Mammary Gland Chemoprevention.

Frontiers in pharmacology

Gautam S,Rawat AK,Sammi SR,Roy S,Singh M,Devi U,Yadav RK,Singh L,Rawat JK,Ansari MN,Saeedan AS,Kumar D,Pandey R,Kaithwas G

Published figure using LOX polyclonal antibody (Product # PA1-16953) in Western Blot

Thu Mar 14 00:00:00 UTC 2024

Defective signaling, osteoblastogenesis and bone remodeling in a mouse model of connexin 43 C-terminal truncation.

Journal of cell science

Moorer MC,Hebert C,Tomlinson RE,Iyer SR,Chason M,Stains JP

Published figure using LOX polyclonal antibody (Product # PA1-16953) in Western Blot

Wed Feb 01 00:00:00 UTC 2017

Elevated ischaemia-associated lysyl oxidase activity in delayed graft failure 6-12 months after renal transplantation.

Experimental physiology

Chen Z,Li Y,Xu H,Ma F,Li J,Zhao L,Xu Y

Published figure using LOX polyclonal antibody (Product # PA1-16953) in Western Blot

Wed Feb 01 00:00:00 UTC 2017

The Nox1/4 Dual Inhibitor GKT137831 or Nox4 Knockdown Inhibits Angiotensin-II-Induced Adult Mouse Cardiac Fibroblast Proliferation and Migration. AT1 Physically Associates With Nox4.

Journal of cellular physiology

Somanna NK,Valente AJ,Krenz M,Fay WP,Delafontaine P,Chandrasekar B

Published figure using LOX polyclonal antibody (Product # PA1-16953) in Western Blot

Sun May 01 00:00:00 UTC 2016

生物信息学

蛋白别名: Lysyl oxidase; Lysyl oxidase (an H-rev gene with its expression down-regulated in HRAS-transformed rat 208F fibroblasts); MGC105112; Protein-lysine 6-oxidase; Protein-lysine 6-oxidase, long form; Protein-lysine 6-oxidase, short form; Ras excision protein; ras recision

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基因别名: AI893619; H-rev142; LOX; Rrg; Rrg1; TSC-160

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UniProt ID: (Human) P28300, (Rat) P16636, (Mouse) P28301

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Entrez Gene ID: (Pig) 100525278, (Human) 4015, (Rat) 24914, (Mouse) 16948

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protein-lysine 6-oxidase activity copper ion binding protein binding oxidoreductase activity oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor metal ion binding

blood vessel development response to hormone collagen fibril organization lung development wound healing response to drug elastic fiber assembly response to steroid hormone oxidation-reduction process cellular protein modification process heart development extracellular matrix organization aorta development