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LPCAT4 Antibody (PA5-89748) in WB
Western blot analysis of LPCAT4 in extracts of various cell lines. Samples were incubated with LPCAT4 Polyclonal antibody (Product # PA5-89748) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-89748
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 345-524 of human LPCAT4 (NP_7058412)
Conjugate
Form
Liquid
Concentration
3.48 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805757
Product Specific Information
Immunogen sequence: AGAEPGRSRM ISQEEFARQL QLSDPQTVAG AFGYFQQDTK GLVDFRDVAL ALAALDGGRS LEELTRLAFE LFAEEQAEGP NRLLYKDGFS TILHLLLGSP HPAATALHAE LCQAGSSQGL SLCQFQNFSL HDPLYGKLFS TYLRPPHTSR GTSQTPNASS PGNPTALANG TVQAPKQKGD; Positive Samples: HT-29, HeLa, HepG2, Mouse brain, Mouse testis; Cellular Location: Endoplasmic reticulum membrane, Multi-pass membrane protein
Target Information
Members of the 1-acylglycerol-3-phosphate O-acyltransferase family, such as AGPAT7, catalyze the conversion of lysophosphatidic acid (LPA) to phosphatidic acid (PA), a precursor in the biosynthesis of all glycerolipids. Both LPA and PA are involved in signal transduction.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: -acylglycerol-3-phosphate O-acyltransferase 7 (lysophosphatidic acid acyltransferase, eta); 1-acylglycerol-3-phosphate O-acyltransferase 7; 1-acylglycerol-3-phosphate O-acyltransferase 7 (lysophosphatidic acid acyltransferase, eta); 1-acylglycerophosphocholine O-acyltransferase; 1-acylglycerophosphoserine O-acyltransferase; 1-AGP acyltransferase 7; 1-AGPAT 7; 1-alkenylglycerophosphoethanolamine O-acyltransferase; 1-alkylglycerophosphocholine O-acetyltransferase; acyl-CoA:lysophosphatidylethanolamine acyltransferase 2; acyltransferase like 3; Acyltransferase-like 3; Lysophosphatidylcholine acyltransferase 4; Lysophosphatidylethanolamine acyltransferase 2; Lysophospholipid acyltransferase LPCAT4; Plasmalogen synthase; PLSC domain containing protein
Gene Aliases: AGPAT7; AI505034; AYTL3; LPAAT-eta; LPCAT4; LPEAT2
UniProt ID: (Human) Q643R3, (Mouse) Q6NVG1
Entrez Gene ID: (Human) 254531, (Mouse) 99010
1-acylglycerol-3-phosphate O-acyltransferase activity
2-acylglycerol-3-phosphate O-acyltransferase activity
1-alkenylglycerophosphoethanolamine O-acyltransferase activity
1-acylglycerophosphocholine O-acyltransferase activity
1-alkylglycerophosphocholine O-acetyltransferase activity
lysophospholipid acyltransferase activity
transferase activity
transferase activity, transferring acyl groups
acyltransferase
phospholipid metabolic process
phosphatidic acid biosynthetic process
phospholipid biosynthetic process
triglyceride biosynthetic process
phosphatidylglycerol acyl-chain remodeling
phosphatidylserine acyl-chain remodeling
phosphatidylcholine acyl-chain remodeling
phosphatidylethanolamine acyl-chain remodeling
lipid metabolic process
metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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