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LPGAT1 Antibody (PA5-103561) in ICC/IF
Immunocytochemistry analysis of LPGAT1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with LPGAT1 polyclonal antibody (Product # PA5-103561) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-103561
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human LPGAT1(Accession Q92604), corresponding to amino acid residues H144-T194.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2852897
Product Specific Information
Antibody detects endogenous levels of total LPGAT1.
Target Information
LPGAT1 is a lysophoshatidylglycerol (LPG) specific acyltransferase that recognizes various acyl-CoAs and LPGs as substrates but demonstrates a clear preference for long chain saturated fatty acyl-CoAs and oleoyl-CoA as acyl donors. It prefers oleoyl-LPG over palmitoyl-LPG as an acyl receptor and oleoyl-CoA over lauroyl-CoA as an acyl donor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2-acylglycerophosphocholine O-acyltransferase; Acyl-CoA:lysophosphatidylglycerol acyltransferase 1; Acyl-CoA:monoacylglycerol acyltransferase LPGAT1; family with sequence similarity 34, member A; LPLAT7; Lysophospholipid acyltransferase 7; Stearoyl-CoA:1-lyso-2-acyl-PE acyltransferase
Gene Aliases: AI649174; AW112037; BC013667; FAM34A; FAM34A1; KIAA0205; LPGAT1; NET8
UniProt ID: (Human) Q92604, (Mouse) Q91YX5
Entrez Gene ID: (Human) 9926, (Mouse) 226856
molecular_function
1-acylglycerol-3-phosphate O-acyltransferase activity
2-acylglycerol-3-phosphate O-acyltransferase activity
dihydrolipoamide branched chain acyltransferase activity
sterol O-acyltransferase activity
N-acetyltransferase activity
O-acyltransferase activity
palmitoleoyl [acyl-carrier-protein]-dependent acyltransferase activity
carnitine O-acyltransferase activity
acetyltransferase activity
C-acyltransferase activity
palmitoyltransferase activity
N-acyltransferase activity
acylglycerol O-acyltransferase activity
serine O-acyltransferase activity
O-acetyltransferase activity
O-octanoyltransferase activity
octanoyltransferase activity
O-palmitoyltransferase activity
S-acyltransferase activity
S-acetyltransferase activity
S-malonyltransferase activity
malonyltransferase activity
C-palmitoyltransferase activity
transferase activity
transferase activity, transferring acyl groups
succinyltransferase activity
N-succinyltransferase activity
O-succinyltransferase activity
S-succinyltransferase activity
sinapoyltransferase activity
O-sinapoyltransferase activity
peptidyl-lysine N6-myristoyltransferase activity
peptidyl-lysine N6-palmitoyltransferase activity
benzoyl acetate-CoA thiolase activity
3-hydroxybutyryl-CoA thiolase activity
3-ketopimelyl-CoA thiolase activity
N-palmitoyltransferase activity
myristoyltransferase activity
acyl-CoA N-acyltransferase activity
protein-cysteine S-myristoyltransferase activity
protein-cysteine S-acyltransferase activity
dihydrolipoamide S-acyltransferase activity
glucosaminyl-phosphotidylinositol O-acyltransferase activity
ergosterol O-acyltransferase activity
lanosterol O-acyltransferase activity
naphthyl-2-oxomethyl-succinyl-CoA succinyl transferase activity
2,4,4-trimethyl-3-oxopentanoyl-CoA 2-C-propanoyl transferase activity
2-methylhexanoyl-CoA C-acetyltransferase activity
butyryl-CoA 2-C-propionyltransferase activity
2,6-dimethyl-5-methylene-3-oxo-heptanoyl-CoA C-acetyltransferase activity
L-2-aminoadipate N-acetyltransferase activity
UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase activity
keto acid formate lyase activity
Ras palmitoyltransferase activity
azetidine-2-carboxylic acid acetyltransferase activity
peptidyl-lysine N-acetyltransferase activity, acting on acetyl phosphate as donor
acetyl-CoA:L-lysine N6-acetyltransferase
acyltransferase
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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