Invitrogen

LRP5 Polyclonal Antibody

查看其他7个LRP5抗体

抗体检测数据 (3)

LRP5 Antibody in Immunocytochemistry (ICC/IF)

LRP5 Antibody in Immunocytochemistry (ICC/IF)

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LRP5 Antibody (PA5-87357) in ICC/IF

Immunofluorescence analysis of LRP5 in NIH/3T3 cells. Samples were incubated with LRP5 Polyclonal antibody (Product # PA5-87357) using a dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

LRP5 Antibody in Immunocytochemistry (ICC/IF)

LRP5 Antibody in Immunocytochemistry (ICC/IF)

LRP5 Antibody in Western Blot (WB)

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LRP5 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of LRP5 in NIH/3T3 cells. Samples were incubated with LRP5 Polyclonal antibody (Product # PA5-87357) using a dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

LRP5 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of LRP5 in PC-12 cells. Samples were incubated with LRP5 Polyclonal antibody (Product # PA5-87357) using a dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

LRP5 Antibody in Western Blot (WB) — Western blot analysis of LRP5 in extracts of various cell lines. Samples were incubated with LRP5 Polyclonal antibody (Product # PA5-87357) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

产品信息

PA5-87357

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:500

-

免疫细胞化学 (ICC/IF)

1:50-1:200

-

酶联免疫吸附实验 (ELISA)

1 µg/mL

-

产品规格

种属反应

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence within amino acids 1400-1500 of human LRP5 (NP_0023262)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.69 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2804089

产品详细信息

Positive Samples: Mouse liver, Rat liver; Cellular Location: Endoplasmic reticulum, Membrane, Single-pass type I membrane protein

Immunogen sequence: YAGANGPFPH EYVSGTPHVP L

靶标信息

LDL related proteins, LRP 5 and LRP 6, are members of the LDL receptor superfamily, which were found to be involved in the canonical Wnt signaling pathway. Mammalian LRP 6 was shown to bind to Wnt 1 and enhance Wnt-induced developmental processes in Xenopus embryos. Mice lacking LRP 6 exhibited developmental defects that are similar to those caused by deficiencies in various Wnt proteins. Recent work also shows that Wnt proteins induce the binding of LRP 5 to Axin, leading Axin degradation and beta catenin stabilization.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: HGNC:6697; Low-density lipoprotein receptor-related protein 5; Low-density lipoprotein receptor-related protein 7; LRP-5; LRP-7

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基因别名: BMND1; HBM; LR3; Lrp5; LRP7; mKIAA4142; OPPG

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UniProt ID: (Mouse) Q91VN0

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Entrez Gene ID: (Rat) 293649, (Mouse) 16973

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molecular_function coreceptor activity Wnt-protein binding toxin transporter activity Wnt-activated receptor activity protein binding transmembrane signal receptor

gastrulation with mouth forming second vasculature development positive regulation of mesenchymal cell proliferation osteoblast development glucose catabolic process cholesterol metabolic process regulation of blood pressure positive regulation of cell proliferation anterior/posterior pattern specification Wnt signaling pathway regulation of ossification embryonic limb morphogenesis positive regulation of osteoblast proliferation somatic stem cell population maintenance limb morphogenesis extracellular matrix-cell signaling cell migration involved in gastrulation cholesterol homeostasis embryonic digit morphogenesis regulation of apoptotic process response to peptide hormone Wnt signaling pathway involved in dorsal/ventral axis specification regulation of fat cell differentiation positive regulation of fat cell differentiation negative regulation of osteoblast differentiation positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter bone remodeling regulation of bone remodeling bone marrow development embryonic camera-type eye morphogenesis positive regulation of sequence-specific DNA binding transcription factor activity anatomical structure regression retina morphogenesis in camera-type eye embryonic retina morphogenesis in camera-type eye canonical Wnt signaling pathway bone development bone morphogenesis branching involved in mammary gland duct morphogenesis mammary gland duct morphogenesis adipose tissue development cell-cell signaling involved in mammary gland development regulation of canonical Wnt signaling pathway regulation of insulin secretion involved in cellular response to glucose stimulus retina vasculature morphogenesis in camera-type eye retinal blood vessel morphogenesis negative regulation of protein serine/threonine kinase activity apoptotic process involved in patterning of blood vessels endocytosis multicellular organism development