Invitrogen

LRRK1 Polyclonal Antibody

3个已发表图片

3 篇参考文献

查看其他12个LRRK1抗体

LRRK1 Antibody in Western Blot (WB)

LRRK1 Antibody in Western Blot (WB)

图: 1 / 5

LRRK1 Antibody (PA5-13868) in WB

Western blot analysis of LRRK1 in mouse bladder tissue lysates. Samples were incubated with LRRK1 polyclonal antibody (Product # PA5-13868). Lysates: 35 µg/lane. LRRK1 (arrow).

LRRK1 Antibody in Western Blot (WB)

LRRK1 Antibody in Western Blot (WB)

LRRK1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

LRRK1 Antibody in Western Blot (WB)

Product Image Gallery

LRRK1 Antibody in Western Blot (WB) — Western blot analysis of LRRK1 in mouse bladder tissue lysates. Samples were incubated with LRRK1 polyclonal antibody (Product # PA5-13868). Lysates: 35 µg/lane. LRRK1 (arrow).

LRRK1 Antibody in Western Blot (WB) — Published figure using LRRK1 polyclonal antibody (Product # PA5-13868) in Western Blot

LRRK1 Antibody in Western Blot, Immunoprecipitation (WB, IP) — Published figure using LRRK1 polyclonal antibody (Product # PA5-13868) in Western Blot and Immunoprecipitation

LRRK1 Antibody in Western Blot (WB) — Published figure using LRRK1 polyclonal antibody (Product # PA5-13868) in Western Blot

LRRK1 Antibody — FIH-1 forms a complex with LRRK1 that affects EGFR activation and keratinocyte migration. A and B: Interaction of LRRK1 with FIH-1. Complex formation of ectopically overexpressed (A) or endogenous (B) FIH-1 with endogenous LRRK1 in HEKs was detected by immunoprecipitation with anti-LRRK1 antibodies, followed by immunoblot analysis with anti-FIH-1 antibodies. C: Interaction of endogenous LRRK1 with endogenous EGFR. Complex formation in HEKs retrovirally transduced with either FIH-1 or empty vector (LZRS) was detected by IP with anti-LRRK1 antibodies, followed by immunoblot analysis with anti-EGFR antibodies. Radioimmunoprecipitation assay-soluble lysates were also immunoblot-analyzed for total EGFR, FIH-1, and GAPDH. D-G: Limbal-derived corneal epithelial hTCEpi cells (D and F) and HEKs (E and G) were transfected with an siRNA pool against either LRRK1 (siLRRK1) or a scrambled control (siCTRL). Seventy-two hours after transfection, immunoblot analysis was performed using antibodies against LRRK1, p-EGFR, GAPDH, and p-ERK (D and E) and scratch-wound-healing assays were conducted (F and G). H and I: hTCEpi cells were transfected with siRNA pools against siFIH-1, siLRRK1, siFIH-1+siLRRK1, or scrambled control (siControl). Seventy-two hours after transfection, immunoblot analyses were performed using antibodies against LRRK1, p-EGFR, GAPDH, and FIH-1 (H) and scratch-wound-healing assays were performed (I). Data are means ± SD (F, G, and I). n = 3 (F, G, and I). *P < 0.05 (F and G) {KD}

产品信息

PA5-13868

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看2篇已发表文章 2篇已发表文章

免疫沉淀 (IP)

-

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 1981-2015 amino acids from the C-terminal region of human LRRK1

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

纯化类型

Size-exclusion - Dialysis, Ammonium sulfate precipitation

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2297136

靶标信息

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Age-Dependent Dopaminergic Neurodegeneration and Impairment of the Autophagy-Lysosomal Pathway in LRRK-Deficient Mice.

Neuron

Giaime E,Tong Y,Wagner LK,Yuan Y,Huang G,Shen J

Published figure using LRRK1 polyclonal antibody (Product # PA5-13868) in Western Blot

Wed Nov 15 00:00:00 UTC 2017

FIH-1 disrupts an LRRK1/EGFR complex to positively regulate keratinocyte migration.

The American journal of pathology

Peng H,Kaplan N,Yang W,Getsios S,Lavker RM

PA5-13868 was used in immunoprecipitation and western blot to identify LRRK1 as a target for FIH-1 during epithelial migration

Mon Dec 01 00:00:00 UTC 2014

FIH-1 disrupts an LRRK1/EGFR complex to positively regulate keratinocyte migration.

The American journal of pathology

Peng H,Kaplan N,Yang W,Getsios S,Lavker RM

PA5-13868 was used in immunoprecipitation and western blot to identify LRRK1 as a target for FIH-1 during epithelial migration

Mon Dec 01 00:00:00 UTC 2014

生物信息学

蛋白别名: leucine-rich repeat kinase 1; Leucine-rich repeat serine/threonine-protein kinase 1; LRRK1

查看更多收起

基因别名: AW319595; C230002E15Rik; D130026O16Rik; KIAA1790; LRRK1; mKIAA1790; RIPK6; Roco1

查看更多收起

UniProt ID: (Human) Q9H5S3, (Mouse) Q3UHC2

查看更多收起

Entrez Gene ID: (Human) 79705, (Mouse) 233328

查看更多收起

protein serine/threonine kinase activity protein binding ATP binding GTP binding identical protein binding metal ion binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase scaffold/adaptor protein

protein phosphorylation small GTPase mediated signal transduction osteoclast development bone resorption positive regulation of peptidyl-tyrosine phosphorylation negative regulation of peptidyl-tyrosine phosphorylation positive regulation of canonical Wnt signaling pathway positive regulation of intracellular signal transduction phosphorylation