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LSM8 Antibody (PA5-144015) in ICC/IF
Immunocytochemistry analysis of LSM8 in A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in LSM8 Polyclonal antibody (Product # PA5-144015) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for... View More
Product Details
PA5-144015
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human LSM8 recombinant protein (Position: M1-H96).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.01mg sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_3075229
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human HELA whole cell, human HL-60 whole cell, human HEPG2 whole cell, human U937 whole cell, human Jurkat whole cell, human Raji whole cell, human THP-1 whole cell, human K562 whole cell, rat testis tissue, rat lung tissue, rat liver tissue, rat pancreas tissue, mouse testis tissue, mouse lung tissue, mouse liver tissue, mouse HEPA1-6 whole cell. IHC: human skin cancer tissue, mouse testis tissue, rat testis tissue, human ovary cancer tissue, human appendicitis tissue, human renal carcinoma tissue, human gastric cancer tissue, human melanoma tissue, human bladder cancer tissue, human tonsil tissue. ICC/IF: A431 cell. Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
This gene encodes a member of the like-Sm family of proteins. The encoded protein consists of a closed barrel shape, made up of five anti-parallel beta strands and an alpha helix. This protein partners with six paralogs to form a heteroheptameric ring which transiently binds U6 small nuclear RNAs and is involved in the general maturation of RNA in the nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: LSM8 homolog, U6 small nuclear RNA associated; LSM8 U6 small nuclear RNA associated; MAK31-like protein; N-alpha-acetyltransferase 38, NatC auxiliary subunit; U6 snRNA-associated Sm-like protein LSm8
Gene Aliases: 2010003I05Rik; AW214405; LSM8; NAA38
UniProt ID: (Human) O95777, (Mouse) Q6ZWM4
Entrez Gene ID: (Human) 51691, (Mouse) 76522, (Rat) 296913
Performance Guarantee
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