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LUM Antibody (PA5-95566) in IHC (P)
Immunohistochemistry analysis of LUM in paraffin-embedded human pancreatic cancer tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with LUM polyclonal antibody (Product # PA5-95566) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex ... View More
Product Details
PA5-95566
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human Lumican recombinant protein (Position: A50-N338).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2807368
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human placenta tissue, human Caco-2 whole cell, human CCRF-CEM whole cell, human Hela whole cell, human Jurkat whole cell, mouse liver tissue, mouse ovary tissue, mouse testis tissue, mouse lung tissue, rat liver tissue, rat ovary tissue, rat testis tissue, rat lung tissue, rat heart tissue. IHC: human ovary cancer tissue, human pancreatic cancer tissue, human lung cancer tissue, human placenta tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
LUM is a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, this protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. The protein is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Keratan sulfate proteoglycan lumican; KSPG lumican; Lumican; Lumican precursor; lumican proteoglycan
Gene Aliases: Lcn; LDC; LUM; SLRR2D
UniProt ID: (Human) P51884, (Rat) P51886, (Mouse) P51885
Entrez Gene ID: (Human) 4060, (Rat) 81682, (Mouse) 17022
visual perception
response to organic cyclic compound
collagen fibril organization
positive regulation of transcription from RNA polymerase II promoter
cartilage development
response to growth factor
axonogenesis
keratan sulfate biosynthetic process
extracellular matrix organization
positive regulation of transforming growth factor beta1 production
keratan sulfate catabolic process
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