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LUM Antibody (MA5-29402) in ICC/IF
Immunofluorescence staining of Human LUM in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with LUM Recombinant Rabbit Monoclonal Antibody (77) (Product # MA5-29402, 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
产品信息
MA5-29402
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
077
抗原
Recombinant Human Lumican Protein (Met1-Asn338)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2785270
产品详细信息
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human Lumican.
靶标信息
LUM is a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, this protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. The protein is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Keratan sulfate proteoglycan lumican; KSPG lumican; Lumican; Lumican precursor; lumican proteoglycan
基因别名: LDC; LUM; SLRR2D
UniProt ID: (Human) P51884
Entrez Gene ID: (Human) 4060
axonogenesis
visual perception
response to organic cyclic compound
keratan sulfate biosynthetic process
extracellular matrix organization
collagen fibril organization
positive regulation of transforming growth factor beta1 production
keratan sulfate catabolic process
positive regulation of transcription from RNA polymerase II promoter
cartilage development
response to growth factor
