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Lamin B1 Antibody (PA5-79607) in ICC/IF
Immunocytochemistry analysis of Lamin B1 using anti-Lamin B1 antibody (Product # PA5-79607) . Lamin B1 was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-Lamin B1 antibody (Product # PA5-79607) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained ... View More
Product Details
PA5-79607
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human Lamin B1 recombinant protein (Position: Q266-C583).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746722
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human U2OS whole cell, human THP-1 whole cell, human A431 whole cell, human RT4 whole cell, rat brain tissue, rat lung tissue, mouse brain tissue, mouse lung tissue. IHC: human stomach cancer tissue, human glioblastoma tissue, human colorectal adenocarcinoma tissue, human breast cancer tissue, human urothelial carcinoma of the bladder with squamous differentiation tissue, mouse cerebellum tissue, rat cerebellum tissue. ICC/IF: U2OS cell IP: Hela cell. Flow: A431 cell.
Target Information
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Defects in the LMNB1 gene can cause Leukodystrophy, demyelinating, autosomal dominant, adult-onset (ADLD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Lamin-B1; MGC111419
Gene Aliases: ADLD; LMN; LMN2; LMNB; LMNB1
UniProt ID: (Human) P20700, (Mouse) P14733, (Rat) P70615
Entrez Gene ID: (Human) 4001, (Mouse) 16906, (Rat) 116685
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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