Invitrogen

Lamin B1 Polyclonal Antibody

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Lamin B1 Antibody (PA5-95850) in WB

Western blot analysis of Lamin B1 in extracts of various cell lines. Samples were incubated with Lamin B1 Polyclonal antibody (Product # PA5-95850) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

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产品信息

PA5-95850

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:5,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:50-1:200

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 503-586 of human Lamin B1 (NP_0055641)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

3.03 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.01% thimerosal

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2807652

产品详细信息

Positive Samples: Jurkat, HT-29, HeLa, U-251MG, mouse liver, mouse brain, mouse spleen, rat liver

Immunogen sequence: AGVTASPPTD LIWKNQNSWG TGEDVKVILK NSQGEEVAQR STVFKTTIPE EEEEEEEAAG VVVEEELFHQ QGTPRASNRS CAIM

靶标信息

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Defects in the LMNB1 gene can cause Leukodystrophy, demyelinating, autosomal dominant, adult-onset (ADLD).

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。