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Laminin beta-1 Antibody (PA5-121243) in WB
Western blot analysis of Laminin beta-1 in various lysates. Samples were incubated with Laminin beta-1 Polyclonal antibody (Product # PA5-121243) using a dilution of 1:2,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
产品信息
PA5-121243
产品规格
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1467-1786 of human Laminin beta 1 (NP_002282.2).
偶联物
形式
Liquid
浓度
0.52 mg/mL
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2914815
产品详细信息
Positive test controls include: SW480, HepG2, HT-1080, SKOV3, Mouse heart. The target is usually found in the following locations: Secreted, basement membrane, extracellular matrix, extracellular space.
Immunogen sequence: EAKLRADEAK QSAEDILLKT NATKEKMDKS NEELRNLIKQ IRNFLTQDSA DLDSIEAVAN EVLKMEMPST PQQLQNLTED IRERVESLSQ VEVILQHSAA DIARAEMLLE EAKRASKSAT DVKVTADMVK EALEEAEKAQ VAAEKAIKQA DEDIQGTQNL LTSIESETAA SEETLFNASQ RISELERNVE ELKRKAAQNS GEAEYIEKVV YTVKQSAEDV KKTLDGELDE KYKKVENLIA KKTEESADAR RKAEMLQNEA KTLLAQANSK LQLLKDLERK YEDNQRYLED KAQELARLEG EVRSLLKDIS QKVAVYSTCL
靶标信息
Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta, and gamma chains. They are ubiquitously present in basement membrane (BM) along with entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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