Invitrogen

Lass5 Polyclonal Antibody

Lass5 Antibody in Immunocytochemistry (ICC/IF)

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Lass5 Antibody (PA5-20570) in ICC/IF

Immunofluorescence of LASS5 in Human Brain cells with Lass5 Polyclonal Antibody (Product # PA5-20570) at 20 µg/mL.

Lass5 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Lass5 Antibody — Fig. 4. Dexamethasone-activated hepatic ceramide synthetic program was attenuated in Angptl4 -/- mice. WT and Angptl4 -/- mice were treated with PBS or dexamethasone for 7 days. ( A to C ) The concentrations of 16 different ceramide species in the liver (A), 5 different free fatty acids (FFAs) in plasma (B), and 4 ceramide species in plasma (C) of these mice were measured. n = 4 mice per group. ( D ) The expression of genes encoding enzymes involved in ceramide production was monitored using quantitative polymerase chain reaction (qPCR). The heat map shows the relative expression compared to that in the WT PBS group. Red shading on the heat map indicates higher expression, and green shading represents lower expression. The changes in fold induction are shown in fig. S1 . n = 16 mice per group. ( E ) Schematic representation of ceramide synthesis pathways. The genes and metabolites that were induced by dexamethasone in WT mice liver are shown in red. The metabolites that were reduced by dexamethasone are shown in green. ( F ) The abundance of Cers5 and Cers6 proteins was monitored using Western blot. The bar graph represents the average intensity of bands normalized to those for Gapdh. Error bars represent SEM. n = 3 to 4 mice per group. * P < 0.05. {TM}

产品信息

PA5-20570

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

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免疫组化 (IHC)

2.5 µg/mL

免疫细胞化学 (ICC/IF)

20 µg/mL

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

An 18 amino acid peptide near the amino terminus of the human LASS5.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_11152546

产品详细信息

A suggested positive control is rat brain tissue lysate.

PA5-20570 can be used with blocking peptide PEP-0690.

靶标信息

The LASS (longevity assurance homolog) family members represent a subgroup of the homeobox gene family and are highly conserved from yeasts to mammals. Six members of this family of proteins have been characterized (LASS1-6) and all are involved in ceramide synthesis during cell growth regulation and cancer differentiation. LASS5, also called Trh4, is a 392 amino acid endoplasmic reticulum, multi-pass membrane protein. Functioning as a dihydro-ceramide synthase, LASS5 is involved in the production of sphingolipids containing mainly one fatty acid donor (N-linked palmitoyl-ceramide) in a fumonisin B1-independent manner. It uses palmitoyl-CoA as an acyl donor and is involved in the synthesis of C14, C16 and C18-ceramide. LASS5 is the most abundantly expressed and predominant ceramide synthase isoform in lung epithelia. Recent studies show that LASS5 partially correct growth and apoptosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The glucocorticoid-Angptl4-ceramide axis induces insulin resistance through PP2A and PKCζ.

Science signaling

Chen TC,Benjamin DI,Kuo T,Lee RA,Li ML,Mar DJ,Costello DE,Nomura DK,Wang JC

Published figure using Lass5 polyclonal antibody (Product # PA5-20570) in Western Blot

Tue Jul 25 00:00:00 UTC 2017

生物信息学

蛋白别名: Ceramide synthase 5; CerS5; LAG1 homolog, ceramide synthase 5; LAG1 longevity assurance homolog 5; longevity assurance homolog 5; Sphingoid base N-palmitoyltransferase CERS5; Sphingosine N-acyltransferase CERS5; TRAM homolog 4; Translocating chain-associating membrane protein homolog 4

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基因别名: 2310081H14Rik; AU045339; AW544927; CERS5; LASS5; Trh4

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UniProt ID: (Human) Q8N5B7, (Mouse) Q9D6K9

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Entrez Gene ID: (Human) 91012, (Mouse) 71949, (Rat) 366984

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功能

DNA binding sphingosine N-acyltransferase activity transferase activity

参与通路

lipid metabolic process sphingolipid metabolic process sphingolipid biosynthetic process ceramide biosynthetic process