Invitrogen

Latexin Recombinant Rabbit Monoclonal Antibody (101)

Latexin Antibody in Immunocytochemistry (ICC/IF)

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Latexin Antibody (MA5-29406) in ICC/IF

Immunofluorescence staining of Human Latexin in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Latexin Recombinant Rabbit Monoclonal Antibody (101) (Product # MA5-29406, 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Latexin Antibody in Flow Cytometry (Flow)

Latexin Antibody in Immunoprecipitation (IP)

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产品信息

MA5-29406

应用

建议稀释比

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免疫印迹 (WB)

1:500-1:2,000

免疫细胞化学 (ICC/IF)

1:20-1:100

流式细胞分析 (Flow)

1:25-1:100

酶联免疫吸附实验 (ELISA)

1:5,000-1:10,000

免疫沉淀 (IP)

4-6 µL/mg of lysate

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

101

抗原

Recombinant Human Latexin/LXN Protein (Glu2-Glu222)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2785273

产品详细信息

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

This antibody has specificity for Human Latexin/LXN.