Invitrogen

Leptin Receptor Polyclonal Antibody

Leptin Receptor Antibody in Western Blot (WB)

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Leptin Receptor Antibody (PA1-053) in WB

Western blot detection of both short and long forms of OB-R from mouse lung tissue extract using Product # PA1-053.

Leptin Receptor Antibody in Immunohistochemistry (IHC)

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产品信息

PA1-053

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

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免疫组化 (IHC)

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免疫组织化学（多聚甲醛固定） (IHC (PFA))

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中和实验 (Neu)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues K(577) E I Q W K T H E V F D A K S K S A(594) of the extracellular domain of rat OB-R.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

10 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

PBS

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2296991

产品详细信息

PA1-053 detects leptin (OB-R) from human, mouse, and rat tissues.

PA1-053 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~100 kDa and ~125 kDa protein representing the short and long forms of OB-R from mouse brain extract. This antibody also detects two smaller, unrelated proteins of ~31 kDa and 35 kDa which are unable to be competed with the peptide in mouse liver extract.

The PA1-053 immunogen is a synthetic peptide corresponding to residues K(577) E I Q W K T H E V F D A K S K S A(594) of the extracellular domain of rat OB-R. This sequence is completely conserved in mouse OB-R. PA1-053 immunizing peptide (Cat. # PEP-014) is available for use in neutralization and control experiments.

靶标信息

The leptin (OB-R) receptor is a member of the cytokine receptor superfamily which plays an important role in mammalian body weight homeostasis and energy balance. Ligand-induced activation of the OB-R by the adipose tissue-secreted hormone, leptin, appears to activate downstream signaling events through the Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway. Leptin can homo-dimerize OB-R extracellular domains while downstream signaling events are initiated upon homo-dimerization of OB-R intracellular domains. Several isoforms of the OB-R which differ in length and signaling capabilities have been reported. RNA transcripts encoding the OB-R long form have been found in the choroid plexus and in the hypothalamus which has been proposed as a control center for satiety and energy expenditure. Mutations in either the mouse OB-R (db) or leptin (ob) genes have been shown to result in early-onset obesity. Metabolic abnormalities attributable to these genotypes include hypercorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered CNS activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Obesity Contributes to Transformation of Myometrial Stem-Cell Niche to Leiomyoma via Inducing Oxidative Stress, DNA Damage, Proliferation, and Extracellular Matrix Deposition.

Genes

Afrin S,Kirschen GW,Borahay MA

PA1-053 was used in Western Blot to determine how adipocytes and adipocyte-secreted factors affect changes in myometrial stem cells (MSCs) in a manner that promotes the growth of uterine leiomyomas.

Tue Aug 15 00:00:00 UTC 2023

Studies of ApoD^-/- and ApoD^-/-ApoE^-/- mice uncover the APOD significance for retinal metabolism, function, and status of chorioretinal blood vessels.

Cellular and molecular life sciences : CMLS

El-Darzi N,Mast N,Petrov AM,Dao T,Astafev AA,Saadane A,Prendergast E,Schwarz E,Bederman I,Pikuleva IA

PA1-053 was used in Immunohistochemistry (PFA fixed) to suggest that the APOD roles in the retina are context specific and could determine retinal glucose fluxes into different pathways.

Mon Feb 01 00:00:00 UTC 2021

Impact of Leptin on Periodontal Ligament Fibroblasts during Mechanical Strain.

International journal of molecular sciences

Schröder A,Meyer A,Spanier G,Damanaki A,Paddenberg E,Proff P,Kirschneck C

PA1-053 was used in Western Blotting to expose PDLF to mechanical strain with or without different leptin concentrations.

Fri Jun 25 00:00:00 UTC 2021

Renal Metabolic Programming Is Linked to the Dynamic Regulation of a Leptin-Klf15 Axis and Akt/AMPKα Signaling in Male Offspring of Obese Dams.

Endocrinology

Kasper P,Vohlen C,Dinger K,Mohr J,Hucklenbruch-Rother E,Janoschek R,Köth J,Matthes J,Appel S,Dötsch J,Alejandre Alcazar MA

PA1-053 was used in Western Blotting to study the impact of maternal obesity on renal-intrinsic insulin and adipocytokine signalling and on renal function and structure.

Sun Oct 01 00:00:00 UTC 2017

Dysregulation of leptin signaling in Alzheimer disease: evidence for neuronal leptin resistance.

Journal of neurochemistry

Bonda DJ,Stone JG,Torres SL,Siedlak SL,Perry G,Kryscio R,Jicha G,Casadesus G,Smith MA,Zhu X,Lee HG

PA1-053 was used in immunohistochemistry to study the involvement of neuronal leptin resistance in Alzheimer's disease

Wed Jan 01 00:00:00 UTC 2014

Leptin reduces pathology and improves memory in a transgenic mouse model of Alzheimer's disease.

Journal of Alzheimer's disease : JAD

Greco SJ,Bryan KJ,Sarkar S,Zhu X,Smith MA,Ashford JW,Johnston JM,Tezapsidis N,Casadesus G

PA1-053 was used in western blot to investigate the effect of leptin treatment on histopathology and memory capability in Alzheimer disease model

Thu Jul 22 00:00:00 UTC 2010

ObRa and ObRe are differentially expressed in adipose tissue in aged food-restricted rats: effects on circulating soluble leptin receptor levels.

Endocrinology

Gallardo N,Arribas C,Villar M,Ros M,Carrascosa JM,Martínez C,Andrés A

PA1-053 was used in western blot to investigate the expression of ObRa and ObRe in adipose tissue in aged food-restricted .

Tue Nov 01 00:00:00 UTC 2005

Estrogen and raloxifene modulate leptin and its receptor in hypothalamus and adipose tissue from ovariectomized rats.

Endocrinology

Meli R,Pacilio M,Raso GM,Esposito E,Coppola A,Nasti A,Di Carlo C,Nappi C,Di Carlo R

PA1-053 was used in western blot to demonstrate the effect of central and peripheral Ob-Rb expression on estrogen levels.

Thu Jul 01 00:00:00 UTC 2004

Leptin distribution and metabolism in the pregnant rat: transplacental leptin passage increases in late gestation but is reduced by excess glucocorticoids.

Endocrinology

Smith JT,Waddell BJ

PA1-053 was used in western blot to study the transplacental passage of maternal leptin and its status during late pregnancy.

Tue Jul 01 00:00:00 UTC 2003

Expression of leptin and its receptor in the murine ovary: possible role in the regulation of oocyte maturation.

Biology of reproduction

Ryan NK,Woodhouse CM,Van der Hoek KH,Gilchrist RB,Armstrong DT,Norman RJ

PA1-053 was used in immunohistochemistry to investigate the distribution of leptin and its receptor in whole tissue sections of the mouse ovary as well as the corresponding effect on mouse oocyte maturation and changes in oocyte cAMP levels

Wed May 01 00:00:00 UTC 2002

Anti-leptin receptor antibody mimics the stimulation of lipolysis induced by leptin in isolated mouse fat pads.

Journal of lipid research

Kawaji N,Yoshida A,Motoyashiki T,Morita T,Ueki H

PA1-053 was used in blocking/activating experiment to study the effect of anti-leptin receptor antibody in isolated mouse fat pads

Mon Oct 01 00:00:00 UTC 2001

Metabolic responses to leptin in obese db/db mice are strain dependent.

American journal of physiology. Regulatory, integrative and comparative physiology

Harris RB,Mitchell TD,Yan X,Simpson JS,Redmann SM

PA1-053 was used in western blot to demonstrate that metabolic responses to leptin in obesedb/db mice are strain dependent

Sun Jul 01 00:00:00 UTC 2001

A leptin dose-response study in obese (ob/ob) and lean (+/?) mice.

Endocrinology

Harris RB,Zhou J,Redmann SM,Smagin GN,Smith SR,Rodgers E,Zachwieja JJ

PA1-053 was used in western blot to study the different leptin sensitivity in obese (ob/ob) and lean (+/?) mice

Thu Jan 01 00:00:00 UTC 1998

生物信息学

蛋白别名: B219; CD antigen CD295; CD295; CD295 antigen; HuB219; HuB219.2; LEP-R; Leptin receptor; Leptin receptor (fatty); leptin receptor gene-related protein; OB receptor; OB-R

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基因别名: CD295; DB; diabetes; Fa; LEP-R; LEPR; Leprb; LEPRD; LEPROT; Modb1; OB-R; OB-RGRP; obese-like; obl; OBR

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UniProt ID: (Human) P48357, (Rat) Q62959, (Mouse) Q61215

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Entrez Gene ID: (Human) 3953, (Rat) 24536, (Mouse) 16847

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功能

transmembrane signaling receptor activity protein binding leptin receptor activity cytokine receptor activity protein-hormone receptor activity peptide hormone binding identical protein binding transmembrane signal receptor

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