Invitrogen

Ly-49C/I/F/H Monoclonal Antibody (14B11), FITC, eBioscience™

Ly-49C/I/F/H Antibody in Flow Cytometry (Flow)

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Ly-49C/I/F/H Antibody (11-5991-81) in Flow

Staining of C57BL/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) PE (Product # 12-5971-82) and staining buffer (autofluorescence) (left) or 0.125 µg of Anti-Mouse Ly-49C/I/F/H FITC (right). Total viable cells were used for analysis.

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产品信息

11-5991-81

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Syrian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

14B11

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_465339

产品详细信息

Description: The 14B11 monoclonal antibody reacts with a subset of mouse Ly-49 receptors and recognizes a majority of mouse NK cells. This antibody reacts with 3 putative inhibitory members of the Ly-49 family, F, I, and C and one putative activating receptor, Ly-49H. 14B11 does not react with Ly-49A, B, D, or G. It is reported that 14B11 enhances the redirected lysis of FcR+ target cells by IL-2-activated NK cells.

Applications Reported: The 14B11 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 14B11 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

KLRA3 (also known as Ly49C) is a member of the LY49 family of receptors in Natural Killer (NK) cells that bind to major histocompatibility complex (MHC) class 1. These proteins are classified as either activating or inhibitory receptors based on whether they possess an immunoreceptor tyrosine-based inhibitory motif (ITIM) in their cytoplasmic region (for inhibitory receptors), or an immunoreceptor tyrosine-based activation motif (ITAM) that transmits activating signals resulting in phosphorylation of several substrates. KLRA3 is thought to be an inhibitory receptor, recognizing peptide -receptive H-2Kb.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

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Product References

Liver-resident NK cells confer adaptive immunity in skin-contact inflammation.

The Journal of clinical investigation

Peng H,Jiang X,Chen Y,Sojka DK,Wei H,Gao X,Sun R,Yokoyama WM,Tian Z

11-5991 was used in Flow cytometry/Cell sorting to define the transcriptional, phenotypic and functional features of natural killer cells in the liver; showing that liver resident CD49a+DX5- natural killer cells possess memory potential and are derived from HPCs/HSCs rather than bone marrow.

Mon Apr 01 00:00:00 UTC 2013

Differential requirements for IRF-2 in generation of CD1d-independent T cells bearing NK cell receptors.

Journal of immunology (Baltimore, Md. : 1950)

Notake T,Horisawa S,Sanjo H,Miyagawa S,Hida S,Taki S

11-5991 was used in Flow cytometry/Cell sorting to investigate the mechanisms behind the generation and function of NKR(+) T cells.

Tue May 15 00:00:00 UTC 2012

A dynamic flux in natural killer cell subsets as a function of the duration of alcohol ingestion.

Alcoholism, clinical and experimental research

Ballas ZK,Cook RT,Shey MR,Coleman RA

11-5991 was used in Flow cytometry/Cell sorting to investigate the effect of duration of alcohol ingestion on NK cell function, showing a dynamic flux in NK cell subsets as a function of ingestion.

Tue May 01 00:00:00 UTC 2012

Development of murine hepatic NK cells during ontogeny: comparison with spleen NK cells.

Clinical & developmental immunology

Wu X,Chen Y,Wei H,Sun R,Tian Z

11-5991 was used in Flow cytometry/Cell sorting to investigate the phenotype of developing liver NK cells compared to spleen NK cells.

Tue Apr 24 00:00:00 UTC 2012

Distinct MHC class I-dependent NK cell-activating receptors control cytomegalovirus infection in different mouse strains.

The Journal of experimental medicine

Pyzik M,Charbonneau B,Gendron-Pontbriand EM,Babić M,Krmpotić A,Jonjić S,Vidal SM

11-5991 was used in Flow cytometry/Cell sorting to study how multiple activating Ly49 receptors may participate in H-2-dependent recognition of MCMV infection, providing a common mechanism of NK cell-mediated resistance against viral infection.

Mon May 09 00:00:00 UTC 2011

Interleukin-6 contributes to age-related alteration of cytokine production by macrophages.

Mediators of inflammation

Gomez CR,Karavitis J,Palmer JL,Faunce DE,Ramirez L,Nomellini V,Kovacs EJ

11-5991 was used in Flow cytometry/Cell sorting to demonstrate in vitro that Interleukin-6 regulates age-related defects in splenic macrophages through alteration of proinflammatory cytokines.

Thu Nov 04 00:00:00 UTC 2010

Unique receptor repertoire in mouse uterine NK cells.

Journal of immunology (Baltimore, Md. : 1950)

Yadi H,Burke S,Madeja Z,Hemberger M,Moffett A,Colucci F

11-5991-81 was used in Flow Cytometry to define the receptor repertoire and phenotype of uterine NK cells at mid-gestation in mice.

Sat Nov 01 00:00:00 UTC 2008

KSHV targets multiple leukocyte lineages during long-term productive infection in NOD/SCID mice.

The Journal of clinical investigation

Parsons CH,Adang LA,Overdevest J,O'Connor CM,Taylor JR,Camerini D,Kedes DH

11-5991 was used in Flow cytometry/Cell sorting to develop an animal model for Kaposi sarcoma-associated herpesvirus (KSHV) infection.

Sat Jul 01 00:00:00 UTC 2006

生物信息学

蛋白别名: 5E6; cytomegalovirus resistance 1; Killer cell lectin-like receptor 3; Killer cell lectin-like receptor 6; Killer cell lectin-like receptor 8; Ly-49c; Ly-49f; Ly-49h; Lymphocyte antigen 49c; Lymphocyte antigen 49f; Lymphocyte antigen 49h; natural killer cell-associated antigen 2; Nk2.1; T-cell surface glycoprotein Ly-49C; T-cell surface glycoprotein Ly-49F; T-cell surface glycoprotein Ly-49H

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基因别名: 5E6; Cmv-1; Cmv1; Klra3; Klra6; Klra8; Ly-49c; Ly-49f; Ly-49h; Ly49-f; Ly49-h; Ly49C; Ly49F; Ly49H; Ly49u<129>; Nk-2; NK-2.1; Nk2; Nk2.1

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UniProt ID: (Mouse) Q64329, (Mouse) Q60653, (Mouse) Q60682

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Entrez Gene ID: (Mouse) 16634, (Mouse) 16637, (Mouse) 16639

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功能

carbohydrate binding

参与通路

cell adhesion response to virus