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AA Sequence of recombinant protein: GSEVSDKRTC VSLTTQRLPV SRIKTYTITE GSLRAVIFIT KRGLKVCADP QATWVRDVVR SMDRKSNTRN NMIQTKPTGT QQSTNTAVTL TG.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Lymphotactin (XCL1). Anti-Human Lymphotactin (XCL1)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hLymphotactin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Lymphotactin (500-P40) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hLymphotactin.
Western Blot: To detect hLymphotactin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hLymphotactin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P40BT-1MG will be provided as 2 x 500 µg
Lymphotactin, otherwise known as XCL1, is a member of the C-chemokine family that uniquely acts as a chemoattractant for lymphocytes, but not monocytes or neutrophils, suggesting an important regulatory role in lymphocyte trafficking and inflammation. Lymphotactin, shown to signal through chemokine XC receptor 1 (XCR1), is detected at the highest level in the spleen and at low levels in the lungs, small intestine and colon, and is abundantly and selectively expressed by activated T cells, in particular CD8+ T cells, and is also produced by NK cells.
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蛋白别名: ATAC; C motif chemokine 1; chemokine (C motif) ligand 1; Cytokine SCM-1; Lymphotactin; Lymphotaxin; SCM-1-alpha; SCM-1-beta; SCM-1alpha; SCM-1beta; SCM1alpha; SCM1beta; single cysteine motif 1a; small inducible cytokine subfamily C, member 1 (lymphotactin); Small-inducible cytokine C1; XC chemokine ligand 1
基因别名: ATAC; LPTN; LTN; SCM-1; SCM-1a; SCM1; SCM1A; SCYC1; XCL1
UniProt ID: (Human) P47992
Entrez Gene ID: (Human) 6375