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M-CSF Antibody (500-P44BT-25UG) in WB
Western Blot: To detect hM-CSF by Western Blot analysis M-CSF Polyclonal Antibody, Biotin (Product # 500-P44BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P44BT-25UG
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Human M-CSF
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2930549
产品详细信息
AA Sequence of recombinant protein: MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human M-CSF. Anti-Human M-CSF-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hM-CSF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human M-CSF (500-P44) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hM-CSF.
Western Blot: To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P44BT-1MG will be provided as 2 x 500 µg
靶标信息
M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: colony stimulating factor 1 (macrophage); CSF-1; H-MCSF; Lanimostim; M-MCSF; macrophage colony stimulating factor 1; Macrophage colony-stimulating factor 1; MGC31930; PG-M-CSF; Proteoglycan macrophage colony-stimulating factor
基因别名: CSF-1; CSF1; MCSF
UniProt ID: (Human) P09603
Entrez Gene ID: (Human) 1435
ossification
positive regulation of cell-matrix adhesion
osteoclast proliferation
developmental process involved in reproduction
inflammatory response
transmembrane receptor protein tyrosine kinase signaling pathway
cell proliferation
positive regulation of cell proliferation
positive regulation of gene expression
regulation of macrophage derived foam cell differentiation
positive regulation of macrophage derived foam cell differentiation
positive regulation of macrophage chemotaxis
hemopoiesis
cell differentiation
macrophage differentiation
regulation of ossification
osteoclast differentiation
positive regulation of cell migration
positive regulation of cellular protein metabolic process
positive regulation of mononuclear cell proliferation
macrophage colony-stimulating factor signaling pathway
positive regulation of multicellular organism growth
monocyte activation
odontogenesis
positive regulation of odontogenesis of dentin-containing tooth
innate immune response
positive regulation of macrophage differentiation
positive regulation of monocyte differentiation
positive regulation of osteoclast differentiation
positive regulation of protein kinase activity
positive regulation of Ras protein signal transduction
homeostasis of number of cells within a tissue
branching involved in mammary gland duct morphogenesis
mammary gland fat development
mammary duct terminal end bud growth
positive regulation of macrophage colony-stimulating factor signaling pathway
positive regulation of microglial cell migration
