Invitrogen

M-CSF Polyclonal Antibody, PeproTech®

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M-CSF Antibody (500-P62G-1MG) in WB

Western Blot: To detect Murine M-CSF by Western Blot analysis M-CSF Polyclonal Antibody (Product # 500-P62G-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine M-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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产品信息

500-P62G-1MG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

- -

酶联免疫吸附实验 (ELISA)

0.5-2.0 µg/mL

产品规格

种属反应

Mouse

宿主/亚型

Goat

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Murine M-CSF

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

AB_2930660

产品详细信息

AA Sequence of recombinant protein: MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP.

Preparation: Produced from sera of goats immunized with highly pure Recombinant Murine M-CSF. Anti-Murine M-CSF-specific antibody was purified by affinity chromatography employing an immobilized Murine M-CSF matrix.

Sandwich ELISA: To detect Murine M-CSF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine M-CSF (500-P62GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine M-CSF.

Western Blot: To detect Murine M-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine M-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

靶标信息

M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CSF-1; H-MCSF; M-MCSF; Macrophage colony-stimulating factor 1; MGC31930; osteopetrosis; PG-M-CSF; Proteoglycan macrophage colony-stimulating factor

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基因别名: C87615; Csf1; Csfm; MCSF; op

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UniProt ID: (Mouse) P07141

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Entrez Gene ID: (Mouse) 12977

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功能

cytokine activity macrophage colony-stimulating factor receptor binding protein binding growth factor activity protein homodimerization activity

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