Invitrogen

M-CSF Polyclonal Antibody

M-CSF Antibody in Immunohistochemistry (IHC)

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M-CSF Antibody (PA5-42558) in IHC

Immunohistochemistry analysis of human placenta tissue using an anti-CSF1 polyclonal antibody (Product # PA5-42558).

M-CSF Antibody in Western Blot, Immunohistochemistry (WB, IHC)

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产品信息

PA5-42558

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.2-1 µg/mL

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免疫组化 (IHC)

Assay-Dependent

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免疫组化（石蜡） (IHC (P))

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产品规格

种属反应

Human

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide directed towards the middle region of human CSF1

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS with 2% sucrose

内含物

0.09% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2609577

产品详细信息

Peptide sequence: SGSVLPLGEL EGRRSTRDRR SPAEPEGGPA SEGAARPLPR FNSVPLTDTG

Sequence homology: Dog: 85%; Guinea Pig: 79%; Human: 100%; Mouse: 86%; Pig: 85%; Rat: 86%

靶标信息

M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Spinal Cord Stimulation Alleviates Neuropathic Pain by Attenuating Microglial Activation via Reducing Colony-Stimulating Factor 1 Levels in the Spinal Cord in a Rat Model of Chronic Constriction Injury.

Anesthesia and analgesia

Sun C,Tao X,Wan C,Zhang X,Zhao M,Xu M,Wang P,Liu Y,Wang C,Xi Q,Song T

PA5-42558 was used in Immunohistochemistry-immunofluorescence to demonstrate that Spinal cord stimulation (SCS) reduces microglial activation in the spinal cord and alleviates chronic neuropathic pain (NP), at least in part by inhibiting the release of colony-stimulating factor 1 (CSF1) from the dorsal root ganglion ipsilateral to nerve injury.

Fri Jul 01 00:00:00 UTC 2022

Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Choi YW,Kim YH,Oh SY,Suh KW,Kim YS,Lee GY,Yoon JE,Park SS,Lee YK,Park YJ,Kim HS,Park SH,Kim JH,Park TJ

PA5-42558 was used in Western Blot, Immunohistochemistry (Paraffin) to suggest senescent tumor cells generate a cytokine barrier protecting nonsenescent tumor cells from immune attack and provide a new target for overcoming the immunotherapy resistance of CRC.

Mon Feb 01 00:00:00 UTC 2021

Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Choi YW,Kim YH,Oh SY,Suh KW,Kim YS,Lee GY,Yoon JE,Park SS,Lee YK,Park YJ,Kim HS,Park SH,Kim JH,Park TJ

Mon Feb 01 00:00:00 UTC 2021

Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Choi YW,Kim YH,Oh SY,Suh KW,Kim YS,Lee GY,Yoon JE,Park SS,Lee YK,Park YJ,Kim HS,Park SH,Kim JH,Park TJ

Mon Feb 01 00:00:00 UTC 2021

Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Choi YW,Kim YH,Oh SY,Suh KW,Kim YS,Lee GY,Yoon JE,Park SS,Lee YK,Park YJ,Kim HS,Park SH,Kim JH,Park TJ

Mon Feb 01 00:00:00 UTC 2021

Schwann cells orchestrate peripheral nerve inflammation through the expression of CSF1, IL-34, and SCF in amyotrophic lateral sclerosis.

Glia

Trias E,Kovacs M,King PH,Si Y,Kwon Y,Varela V,Ibarburu S,Moura IC,Hermine O,Beckman JS,Barbeito L

PA5-42558 was used in Immunohistochemistry-immunofluorescence to analyse schwann cells phenotypes in sciatic nerves of amyotrophic lateral sclerosis patients and paralytic SOD1G93A rats to identify if similar and specific reactive schwann cells phenotypes exist.

Mon Jun 01 00:00:00 UTC 2020

Schwann cells orchestrate peripheral nerve inflammation through the expression of CSF1, IL-34, and SCF in amyotrophic lateral sclerosis.

Glia

Trias E,Kovacs M,King PH,Si Y,Kwon Y,Varela V,Ibarburu S,Moura IC,Hermine O,Beckman JS,Barbeito L

Mon Jun 01 00:00:00 UTC 2020

Schwann cells orchestrate peripheral nerve inflammation through the expression of CSF1, IL-34, and SCF in amyotrophic lateral sclerosis.

Glia

Trias E,Kovacs M,King PH,Si Y,Kwon Y,Varela V,Ibarburu S,Moura IC,Hermine O,Beckman JS,Barbeito L

Mon Jun 01 00:00:00 UTC 2020

Regulators of TNFα mediated insulin resistance elucidated by quantitative proteomics.

Scientific reports

Mohallem R,Aryal UK

PA5-42558 was used in Western Blotting to investigate the changes in the global proteome, secretome and phosphoproteome of adipocytes under chronic inflammation condition, as well as fundamental cross-talks between different cellular pathways regulated by chronic TNFα exposure.

Mon Nov 30 00:00:00 UTC 2020

Regulators of TNFα mediated insulin resistance elucidated by quantitative proteomics.

Scientific reports

Mohallem R,Aryal UK

Mon Nov 30 00:00:00 UTC 2020

生物信息学

蛋白别名: colony stimulating factor 1 (macrophage); CSF-1; H-MCSF; Lanimostim; M-MCSF; macrophage colony stimulating factor 1; Macrophage colony-stimulating factor 1; MGC31930; PG-M-CSF; Proteoglycan macrophage colony-stimulating factor

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基因别名: CSF-1; CSF1; MCSF

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UniProt ID: (Human) P09603

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Entrez Gene ID: (Human) 1435

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功能

cytokine activity macrophage colony-stimulating factor receptor binding protein binding growth factor activity protein homodimerization activity

参与通路