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Mouse IgG2a Isotype Control (MA1-193-D650) in ICC/IF
Imunofluorescent analysis of Lin28 (red) in Human episomal iPSC cells. The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% Blocker BSA (Product # 37525) for 30 minutes at room temperature. Cells were stained with Dylight 650-conjugated Lin28 monoclonal antibody (right, Product # MA1-016-D650) or Dylight 650-conjugated Mouse IgG2a... View More
产品信息
MA1-193-D650
应用
建议稀释比
免疫印迹 (WB)
Assay-dependent
免疫组化（石蜡） (IHC (P))
Assay-dependent
免疫组化（冰冻） (IHC (F))
Assay-dependent
免疫细胞化学 (ICC/IF)
Assay-dependent
流式细胞分析 (Flow)
Assay-dependent
对照试剂 (Ctrl)
Assay-dependent
产品规格
宿主/亚型
Mouse
/ IgG2a, kappa
分类
Monoclonal
类型
Isotype Control
克隆号
MOPC-173
抗原
The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice
偶联物
激发/发射光谱
651/673 nm
查看光谱
形式
Liquid
浓度
0.1 mg/mL
纯化类型
Size-exclusion chromatography
保存液
PBS with 0.2% BSA
内含物
15mM sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2536961
产品详细信息
This antibody reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.
MA1-193-D650 can be used as a negative control in any immunoassay where a DyLight 650-conjugated primary antibody of the mouse IgG2a isotype is used. DyLight 650 has an excitation/emission of 652/672nm.
靶标信息
Mouse IgG2a is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the second most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG2a is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG2a is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
