Invitrogen

CD155 Monoclonal Antibody (D171), Biotin

6 篇参考文献

查看其他34个CD155抗体

抗体检测数据 (5)

CD155 Antibody in Western Blot (WB)

CD155 Antibody in Western Blot (WB)

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CD155 Antibody (MA5-13490) in WB

Western blot analysis of CD155 was performed by loading 25 µg of mouse stomach (Lane 1), U-937 (Lane 2) and Hela (Lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a CD155 monoclonal antibody (Product # MA5-13493) at a dilution of 1:20 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Bl... View More

CD155 Antibody in Western Blot (WB)

CD155 Antibody in Immunocytochemistry (ICC/IF)

CD155 Antibody in Immunocytochemistry (ICC/IF)

CD155 Antibody in Flow Cytometry (Flow)

CD155 Antibody in Flow Cytometry (Flow)

Product Image Gallery

CD155 Antibody in Western Blot (WB) — Western blot analysis of CD155 was performed by loading 25 µg of mouse stomach (Lane 1), U-937 (Lane 2) and Hela (Lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a CD155 monoclonal antibody (Product # MA5-13493) at a dilution of 1:20 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at approx. 45 kDa. This image was obtained using the unconjugated form of the antibody.

CD155 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of CD155 (green) showing staining in the membrane of U-87 MG cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD155 monoclonal antibody (Product # MA5-13493) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

CD155 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of CD155 (green) showing staining in the membrane of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD155 monoclonal antibody (Product # MA5-13493) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

CD155 Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of CD155 in U937 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD155 monoclonal antibody (Product # MA5-13493) at a dilution of 2 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

CD155 Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of CD155 in Hela cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD155 monoclonal antibody (Product # MA5-13493) at a dilution of 2 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

产品信息

MA5-13490

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:10-1:100

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

-

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:10-1:100

查看4篇已发表文章 4篇已发表文章

流式细胞分析 (Flow)

Assay-dependent

-

产品规格

种属反应

Human, Mouse, Non-human primate

已发表种属

Human, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

D171

抗原

HeLa cells

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偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_10980701

产品详细信息

MA5-13490 targets CD155 in FACS, WB and ICC/IF applications and shows reactivity with Human, Mouse and Non-human primate samples.

The MA5-13490 immunogen is heLa cells.

靶标信息

The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Physical Characterization of Colorectal Cancer Spheroids and Evaluation of NK Cell Infiltration Through a Flow-Based Analysis.

Frontiers in immunology

Sargenti A,Musmeci F,Bacchi F,Delprete C,Cristaldi DA,Cannas F,Bonetti S,Pasqua S,Gazzola D,Costa D,Villa F,Zocchi MR,Poggi A

MA5-13490 was used in Immunocytochemistry to employ a flow-based method specifically conceived to measure weight, size and focused onto mass density values of tumor spheroids.

Mon May 03 00:00:00 UTC 2021

Inhibition of Necl-5 (CD155/PVR) reduces glioblastoma dispersal and decreases MMP-2 expression and activity.

Journal of neuro-oncology

Enloe BM,Jay DG

MA5-13490 was used in immunocytochemistry to investigate the effect of Necl-5 blockade on glioblastoma progression and specific gene expression

Fri Apr 01 00:00:00 UTC 2011

NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways.

Molecular immunology

Verhoeven DH,de Hooge AS,Mooiman EC,Santos SJ,ten Dam MM,Gelderblom H,Melief CJ,Hogendoorn PC,Egeler RM,van Tol MJ,Schilham MW,Lankester AC

MA5-13490 was used in immunohistochemistry to investigate the effect of natural killer cells on Ewing sarcoma and its mechanism

Mon Sep 01 00:00:00 UTC 2008

CD155/PVR enhances glioma cell dispersal by regulating adhesion signaling and focal adhesion dynamics.

Cancer research

Sloan KE,Stewart JK,Treloar AF,Matthews RT,Jay DG

MA5-13490 was used in immunocytochemistry to investigate the effect of CD155/PVR on glioma cell adhesion

Thu Dec 01 00:00:00 UTC 2005

Receptor (CD155)-dependent endocytosis of poliovirus and retrograde axonal transport of the endosome.

Journal of virology

Ohka S,Matsuda N,Tohyama K,Oda T,Morikawa M,Kuge S,Nomoto A

MA5-13490 was used in immunocytochemistry to study CD155-dependent endocytosis and retrograde axonal transport of polivirus-containg vessicles

Thu Jul 01 00:00:00 UTC 2004

Identification of secreted CD155 isoforms.

Biochemical and biophysical research communications

Baury B,Masson D,McDermott BM,Jarry A,Blottière HM,Blanchardie P,Laboisse CL,Lustenberger P,Racaniello VR,Denis MG

MA5-13490 was used in western blot to study secreted human CD155 isoforms

Fri Sep 12 00:00:00 UTC 2003

生物信息学

蛋白别名: CD155; FLJ25946; NECL-5; nectin-like 5; Nectin-like protein 5; Poliovirus receptor; sCD112; soluble CD112; tumor-associated antigen 1

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基因别名: 3830421F03Rik; CD155; D7Ertd458e; HVED; mE4; Necl-5; NECL5; PVR; PVS; Taa1; TAGE4

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UniProt ID: (Human) P15151

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Entrez Gene ID: (Human) 5817, (Mouse) 52118

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virus receptor activity receptor activity receptor binding protein binding protein homodimerization activity cell adhesion molecule binding

single organismal cell-cell adhesion cell migration viral entry into host cell positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target homophilic cell adhesion via plasma membrane adhesion molecules heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules cell recognition adherens junction organization susceptibility to natural killer cell mediated cytotoxicity positive regulation of natural killer cell mediated cytotoxicity regulation of immune response susceptibility to T cell mediated cytotoxicity