Invitrogen

VCAM-1 Monoclonal Antibody (1.G11B1)

VCAM-1 Antibody in Immunocytochemistry (ICC/IF)

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VCAM-1 Antibody (MA5-16429) in ICC/IF

Immunofluorescence analysis of VCAM-1 was performed using 70% confluent log phase HAEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with VCAM-1 Mouse monoclonal Antibody (Product # MA5-16429) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at... View More

VCAM-1 Antibody in Flow Cytometry (Flow)

VCAM-1 Antibody in Western Blot, Neutralization, Inhibition Assays (WB, Neu, IA)

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产品信息

MA5-16429

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

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免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫细胞化学 (ICC/IF)

5 µg/ml

流式细胞分析 (Flow)

1:10-1:50

查看3篇已发表文章 3篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

Assay-dependent

染色质免疫沉淀实验 (ChIP)

查看1篇已发表文章 1篇已发表文章

中和实验 (Neu)

查看3篇已发表文章 3篇已发表文章

功能检测 (Functional)

Assay-dependent

抑制试剂 (IA)

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其他PubMed (Misc)

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产品规格

种属反应

Human, Pig, Rhesus monkey

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

1.G11B1

抗原

human endothelial cells

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2537945

产品详细信息

Non-reducing conditions are recommended for Western blot applications.

靶标信息

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Multiplexing Biosensor for the Detection of Extracellular Vesicles as Biomarkers of Tissue Damage and Recovery after Ischemic Stroke.

International journal of molecular sciences

Picciolini S,Mangolini V,Rodà F,Montesano A,Arnaboldi F,Liuzzi P,Mannini A,Bedoni M,Gualerzi A

MA5-16429 was used in ChIP assay to identify a panel of EV-associated molecules that (i) confirm the crucial involvement of EVs in the processes that follow ischemic stroke, (ii) could possibly profile ischemic stroke patients at the beginning of the rehabilitation program, (iii) could be used in predicting patients' response to treatment.

Thu Apr 27 00:00:00 UTC 2023

Mesenchymal-endothelial nexus in breast cancer spheroids induces vasculogenesis and local invasion in a CAM model.

Communications biology

Li A,Muenst S,Hoffman J,Starck L,Sarem M,Fischer A,Hutter G,Shastri VP

MA5-16429 was used in Immunohistochemistry to provide a framework for developing well-defined in vitro systems, including patient-derived xenografts that recapitulate in vivo events, to investigate heterotypic cell interactions in tumors, to identify factors promoting tumor metastasis-related events, and possibly drug screening in the context of personalized medicine.

Sun Nov 27 00:00:00 UTC 2022

The pretubulysin-induced exposure of collagen is caused by endothelial cell retraction that results in an increased adhesion and decreased transmigration of tumor cells.

Oncotarget

Schwenk R,Stehning T,Bischoff I,Ullrich A,Kazmaier U,Fürst R

Published figure using VCAM-1 monoclonal antibody (Product # MA5-16429) in Inhibition Assays

Fri Sep 29 00:00:00 UTC 2017

The pretubulysin-induced exposure of collagen is caused by endothelial cell retraction that results in an increased adhesion and decreased transmigration of tumor cells.

Oncotarget

Schwenk R,Stehning T,Bischoff I,Ullrich A,Kazmaier U,Fürst R

Published figure using VCAM-1 monoclonal antibody (Product # MA5-16429) in Inhibition Assays

Fri Sep 29 00:00:00 UTC 2017

The pretubulysin-induced exposure of collagen is caused by endothelial cell retraction that results in an increased adhesion and decreased transmigration of tumor cells.

Oncotarget

Schwenk R,Stehning T,Bischoff I,Ullrich A,Kazmaier U,Fürst R

Published figure using VCAM-1 monoclonal antibody (Product # MA5-16429) in Inhibition Assays

Fri Sep 29 00:00:00 UTC 2017

Adhesion of human hematopoietic progenitor cells to mesenchymal stromal cells involves CD44.

Cells, tissues, organs

Wagner W,Wein F,Roderburg C,Saffrich R,Diehlmann A,Eckstein V,Ho AD

MA5-16429 was used in blocking or activating experiment to test whether CD44 aids in adhesion of human hematopoietic progenitor cells to mesenchymal stromal cells

Fri Sep 19 00:00:00 UTC 2008

Effects of vascular endothelial growth factor on the lymphocyte-endothelium interactions: identification of caveolin-1 and nitric oxide as control points of endothelial cell anergy.

Journal of immunology (Baltimore, Md. : 1950)

Bouzin C,Brouet A,De Vriese J,Dewever J,Feron O

MA5-16429 was used in blocking or activating experiment to clarify mechanisms that regulate tumor endothelial cells anergy

Thu Feb 01 00:00:00 UTC 2007

Immunological and ultrastructural characterization of endothelial cell cultures differentiated from human cord blood derived endothelial progenitor cells.

Histochemistry and cell biology

Neumüller J,Neumüller-Guber SE,Lipovac M,Mosgoeller W,Vetterlein M,Pavelka M,Huber J

MA5-16429 was used in flow cytometry to assess the ability of endothelial progenitor cells to differentiate into early mature endothelial cells

Fri Dec 01 00:00:00 UTC 2006

Irradiation of mechanically-injured human arterial endothelial cells leads to increased gene expression and secretion of inflammatory and growth promoting cytokines.

Atherosclerosis

Wondergem J,Wedekind LE,Bart CI,Chin A,van der Laarse A,Beekhuizen H

MA5-16429 was used in flow cytometry to assess the effect of irradiation on re-endothelialisation of circular denuded tracks made in post-confluent cultures of arterial endothelial cells and cellular factors that contribute to this process

Thu Jul 01 00:00:00 UTC 2004

Effect of maternal anti-HPA-1a antibodies and polyclonal IVIG on the activation status of vascular endothelial cells.

Clinical and experimental immunology

Radder CM,Beekhuizen H,Kanhai HH,Brand A

MA5-16429 was used in flow cytometry to test if anti-HPA-1a antibodies and intravenous immunoglobulin affect vascular endothelial cells in order to identify susceptibility for intracranial hemorrhage

Thu Jul 01 00:00:00 UTC 2004

Human pulmonary valve endothelial cells express functional adhesion molecules for leukocytes.

The Journal of heart valve disease

Dvorin EL,Jacobson J,Roth SJ,Bischoff J

MA5-16429 was used in flow cytometry to explore how the cardiac valve endothelium is not damaged after transplantation.

Mon Sep 01 00:00:00 UTC 2003

生物信息学

蛋白别名: CD106; CD106 antigen; DKFZp779G2333; INCAM-100; INCAM100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; V-CAM 1; Vascular cell adhesion protein 1

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基因别名: CD106; EGK_01015; INCAM-100; VCAM1

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UniProt ID: (Human) P19320

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Entrez Gene ID: (Rhesus monkey) 712036, (Human) 7412, (Pig) 396925

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功能

integrin binding primary amine oxidase activity cell adhesion molecule binding

参与通路

response to hypoxia acute inflammatory response chronic inflammatory response cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion cell-matrix adhesion aging response to nutrient amine metabolic process response to zinc ion response to ionizing radiation viral process membrane to membrane docking B cell differentiation extracellular matrix organization response to lipopolysaccharide response to nicotine calcium-mediated signaling using intracellular calcium source cellular response to vascular endothelial growth factor stimulus positive regulation of T cell proliferation response to ethanol regulation of immune response leukocyte tethering or rolling oxidation-reduction process cell chemotaxis interferon-gamma-mediated signaling pathway cellular response to tumor necrosis factor

VCAM-1 Monoclonal Antibody (1.G11B1)

VCAM-1 Antibody (MA5-16429) in ICC/IF

产品信息

MA5-16429

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

酶联免疫吸附实验 (ELISA)

免疫沉淀 (IP)

染色质免疫沉淀实验 (ChIP)

中和实验 (Neu)

功能检测 (Functional)

抑制试剂 (IA)

其他PubMed (Misc)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

VCAM-1 Monoclonal Antibody (1.G11B1)

VCAM-1 Antibody (MA5-16429) in ICC/IF

产品信息

MA5-16429

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

酶联免疫吸附实验 (ELISA)

免疫沉淀 (IP)

染色质免疫沉淀 实验 (ChIP)

中和实验 (Neu)

功能检测 (Functional)

抑制试剂 (IA)

其他PubMed (Misc)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)