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产品信息
MA5-24661
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
RM8
抗原
Peptide corresponding to B-Raf V600E mutant.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.2-7.4, with 50% glycerol, 1% BSA
内含物
0.09% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2661889
产品详细信息
This antibody reacts to the BRAF V600E mutant. No cross reactivity with wild type BRAF.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
This gene, B-RAF/BRAF, encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); murine sarcoma viral (v-raf) oncogene homolog B1; p94; Proto-oncogene B-Raf; Serine/threonine-protein kinase B-raf; v-raf murine sarcoma viral oncogene homolog B; v-Raf murine sarcoma viral oncogene homolog B1
基因别名: B-raf; B-RAF1; BRAF; BRAF1; NS7; RAFB1
UniProt ID: (Human) P15056
Entrez Gene ID: (Human) 673
protein kinase activity
protein serine/threonine kinase activity
MAP kinase kinase kinase activity
calcium ion binding
protein binding
ATP binding
small GTPase binding
mitogen-activated protein kinase kinase binding
identical protein binding
protein heterodimerization activity
non-receptor serine/threonine protein kinase
MAPK cascade
activation of MAPKK activity
myeloid progenitor cell differentiation
protein phosphorylation
visual learning
organ morphogenesis
positive regulation of gene expression
negative regulation of fibroblast migration
glucose transport
thyroid gland development
positive regulation of peptidyl-serine phosphorylation
somatic stem cell population maintenance
cellular response to drug
regulation of cell proliferation
negative regulation of apoptotic process
CD4-positive, alpha-beta T cell differentiation
positive T cell selection
response to peptide hormone
negative regulation of neuron apoptotic process
thymus development
positive regulation of axon regeneration
positive regulation of axonogenesis
protein heterooligomerization
positive regulation of stress fiber assembly
response to cAMP
long-term synaptic potentiation
head morphogenesis
face development
positive regulation of ERK1 and ERK2 cascade
cellular response to calcium ion
establishment of protein localization to membrane
positive regulation of substrate adhesion-dependent cell spreading
negative regulation of synaptic vesicle exocytosis
negative regulation of endothelial cell apoptotic process
