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Spectrin beta-4 Antibody (MA5-45721) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with beta 4 Spectrin monoclonal antibody (Product # MA5-45721) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000; 1:5,... View More
产品信息
MA5-45721
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
N393/2
抗原
Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin
偶联物
激发/发射光谱
651/660 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2932175
产品详细信息
A 1:100 dilution of MA5-45721 was sufficient for detection of Beta 4 Spectrin in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |Detects approximately >200kDa. Does not cross react with other Beta-spectrins
This antibody was formerly sold as clone S393-2.
靶标信息
Spectrin is the major constituent of the cytoskeletal network underlying the erthrocyte plasma membrane and determines cell shape, arrangement of transmembrane proteins and organization of organelles. It is expressed at very low levels in many tissues, with the strongest expression in the cerebellum, spinal cord, stomach, pituitary gland, liver, pancreas, salivary gland, kidney, bladder and heart. Spectrin beta 4 is a non-erythrocyte member, and is expressed in the brain and pancreatic islets and localisted to the nuclear matrix, cytoplasmic vesicles and PML nuclear bodies. Beta4 spectrins are also essential for membrane stability and the molecular organization of the nodes of Ranvier.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Beta-IV spectrin; beta-spectrin 4; EMBL BAB83243.1; lumbosacral neuroaxonal dystrophy; neuroaxonal dystrophy; quivering; spectrin beta 4; spectrin beta chain, brain 3; Spectrin beta chain, non-erythrocytic 4; spectrin, beta, non-erythrocytic 4; Spectrin, non-erythroid beta chain 3
基因别名: 1700022P15Rik; 5830426A08Rik; dyn; KIAA1642; lnd; nmf261; nmf379; QV; ROSA62; SpbIV; SPNB4; SPTBN3; SPTBN4
UniProt ID: (Human) Q9H254
Entrez Gene ID: (Human) 57731, (Mouse) 80297, (Rat) 308458
MAPK cascade
regulation of sodium ion transport
ER to Golgi vesicle-mediated transport
cytoskeletal anchoring at plasma membrane
axonogenesis
axon guidance
sensory perception of sound
adult walking behavior
fertilization
negative regulation of heart rate
vesicle-mediated transport
transmission of nerve impulse
central nervous system projection neuron axonogenesis
regulation of peptidyl-serine phosphorylation
positive regulation of multicellular organism growth
positive regulation of GTPase activity
clustering of voltage-gated sodium channels
actin filament capping
cardiac conduction
establishment of protein localization to plasma membrane
reproductive process
adult behavior
cellular protein localization
biological_process
