Invitrogen

MAD2 Polyclonal Antibody

MAD2 Antibody in Immunocytochemistry (ICC/IF)

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MAD2 Antibody (PA5-21594) in ICC/IF

Immunofluorescence analysis of human osteosarcoma cell line U2OS, using MAD2L1 antibody (Product # PA5-21594) at 1:1,000 dilution.

MAD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MAD2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

MAD2 Antibody — Antibody specificity was demonstrated by shRNA mediated knockdown of the target protein. 293T cells were transfected with MAD2 shRNA and decrease in signal intensity was observed in western blot application using MAD2 antibody (Product # PA5-21594). {KD}

产品信息

PA5-21594

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

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免疫沉淀 (IP)

1:100-1:500

产品规格

种属反应

Human, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to a region within amino acids 142 and 205 of MAD2L1 (Uniprot ID#Q13257)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.01 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_11154021

产品详细信息

Recommended positive controls: 293T, NCIN87, U2OS, HepG2, Molt4, Raji.

Predicted reactivity: Rhesus Monkey (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Tight junction proteins (TJPs) belong to a family of membrane-associated guanylate kinase (MAGUK) homologs that are involved in the organization of epithelial and endothelial intercellular junctions. TJPs bind to the cytoplasmic C termini of junctional transmembrane proteins and link them to the actin cytoskeleton.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

BAP1 loss induces mitotic defects in mesothelioma cells through BRCA1-dependent and independent mechanisms.

Oncogene

Singh A,Busacca S,Gaba A,Sheaff M,Poile C,Nakas A,Dzialo J,Bzura A,Dawson AG,Fennell DA,Fry AM

PA5-21594 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to propose that BRCA1-associated protein 1 inactivation causes mitotic defects through BRCA1-dependent and independent mechanisms.

Wed Feb 01 00:00:00 UTC 2023

BAP1 loss induces mitotic defects in mesothelioma cells through BRCA1-dependent and independent mechanisms.

Oncogene

Singh A,Busacca S,Gaba A,Sheaff M,Poile C,Nakas A,Dzialo J,Bzura A,Dawson AG,Fennell DA,Fry AM

Wed Feb 01 00:00:00 UTC 2023

CCAR2 controls mitotic progression through spatiotemporal regulation of Aurora B.

Cell death & disease

Ryu J,Kim JE

Published figure using MAD2 polyclonal antibody (Product # PA5-21594) in Immunocytochemistry

Tue Jun 07 00:00:00 UTC 2022

Phosphorylation and Pin1 binding to the LIC1 subunit selectively regulate mitotic dynein functions.

The Journal of cell biology

Kumari A,Kumar C,Pergu R,Kumar M,Mahale SP,Wasnik N,Mylavarapu SVS

PA5-21594 was used in Western Blotting to reveal that LIC1-CTD phosphorylation differentially regulates distinct mitotic dynein pools and suggests the evolutionary conservation of this phosphoregulation.

Mon Dec 06 00:00:00 UTC 2021

Atypical APC/C-dependent degradation of Mcl-1 provides an apoptotic timer during mitotic arrest.

The EMBO journal

Allan LA,Skowyra A,Rogers KI,Zeller D,Clarke PR

PA5-21594 was used in Western Blotting to show that proteolytic destruction of the key regulator Mcl-1 during an extended mitosis requires the anaphase-promoting complex or cyclosome (APC/C) and is independent of another ubiquitin E3 ligase, SCFFbw7.

Mon Sep 03 00:00:00 UTC 2018

TC Mps1 12, a novel Mps1 inhibitor, suppresses the growth of hepatocellular carcinoma cells via the accumulation of chromosomal instability.

British journal of pharmacology

Choi M,Min YH,Pyo J,Lee CW,Jang CY,Kim JE

PA5-21594 was used in Immunocytochemistry to establish the inhibition of Mps1 kinase using TC Mps1 12 as a therapeutic treatment for liver cancer patients.

Thu Jun 01 00:00:00 UTC 2017

Polo-like kinase 1 inhibitor BI2536 causes mitotic catastrophe following activation of the spindle assembly checkpoint in non-small cell lung cancer cells.

Cancer letters

Choi M,Kim W,Cheon MG,Lee CW,Kim JE

PA5-21594 was used in immunocytochemistry to elucidate the mechanism by which BI2536 induces defects in cell proliferation of non-small cell lung cancer

Sat Feb 28 00:00:00 UTC 2015

生物信息学

蛋白别名: HsMAD2; MAD2 (mitotic arrest deficient, homolog)-like 1; MAD2 (mitotic arrest deficient, yeast, homolog)-like 1; MAD2-like 1; MAD2-like protein 1; MadL1; Mitotic arrest deficient 2-like protein 1; mitotic arrest deficient, yeast, homolog-like 1; Mitotic spindle assembly checkpoint protein MAD2A

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基因别名: HSMAD2; MAD2; MAD2L1

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UniProt ID: (Human) Q13257

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Entrez Gene ID: (Human) 4085, (Rat) 297176

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功能

protein binding identical protein binding protein homodimerization activity

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