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MADD Antibody (A302-144A) in IHC (P)
Detection of human MADD by immunohistochemistry. Sample: FFPE sections of human breast carcinoma and colon carcinoma. Antibody: Affinity purified rabbit anti-MADD (Product # A302-144A) (Lot 1) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB. and Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.
产品信息
A302-144A
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Region between residue 1597 and 1647 of human MAP-kinase activating death domain.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
tris citrate/phosphate, pH 7-8
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Wet ice
产品详细信息
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Based on 100% sequence identity, this antibody is predicted to react with Rat
靶标信息
Tumor necrosis factor receptor 1 (TNFR1) contains a death receptor domain that mediates interactions involved in downstream signaling of TNF. A novel protein, MADD (mitogen activated protein (MAP)-kinase activating death domain protein) has recently been shown to associate with the death domain of TNFR1 through its own C-terminal death domain. This interaction is critical for TNFR1 signal generation. Overexpression of MADD activates the MAP (Mitogen Activated Protein) kinases extracellular signal-related kinase (ERK) and c-Jun N-terminal kinase (JNK) and induces the phosphorylation of cytosolic phospholipase A2. This discovery suggests that MADD links tumor necrosis factor TNF and MAP kinase activation and arachidonic acid release. MADD has a 98% homology with another protein, differentially expressed in neoplasmic vs. normal cells (DENN). DENN is a substrate for JNK3, and is important in the hypoxia and stress induced intracellular signalling pathways. MADD also has a 93% sequence conservation with a GDP/GTP exchange protein, Rab3.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Differentially expressed in normal and neoplastic cells; Insulinoma glucagonoma clone 20; MADD; MAP kinase-activating death domain protein; MAP-kinase activating death domain; Rab3 GDP/GTP exchange factor; Rab3 GDP/GTP exchange protein; Rab3GEP; RabGEF
基因别名: DENN; IG20; KIAA0358; MADD; RAB3GEP
UniProt ID: (Human) B5MEE5
Entrez Gene ID: (Human) 8567
activation of MAPK activity
cell surface receptor signaling pathway
regulation of tumor necrosis factor-mediated signaling pathway
regulation of Rab protein signal transduction
regulation of apoptotic process
positive regulation of GTPase activity
regulation of cell cycle
execution phase of apoptosis
regulation of extrinsic apoptotic signaling pathway via death domain receptors
regulation of extrinsic apoptotic signaling pathway
