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MAN2A1 Antibody (PA5-115539) in ICC/IF
Immunocytochemistry analysis of MAN2A1 in HepG2 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115539) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
产品信息
PA5-115539
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Non-human primate,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human MAN2A1(Accession Q16706), corresponding to amino acid residues Y156-D206.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2900175
产品详细信息
Antibody detects endogenous levels of total Mannosidase II.
靶标信息
This gene encodes a glycosyl hydrolase that localizes to the Golgi and catalyzes the final hydrolytic step in the asparagine-linked oligosaccharide (N-glycan) maturation pathway. Mutations in the mouse homolog of this gene have been shown to cause a systemic autoimmune disease similar to human systemic lupus erythematosus.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Alpha-mannosidase 2; alpha-mannosidase II; AMan II; Golgi alpha-mannosidase II; golgi integral membrane protein 7; MAN II; mannosidase 2, alpha 1; mannosidase alpha 2; Mannosidase alpha class 2A member 1; mannosidase, alpha type II; mannosidase, alpha, class 2A, member 1; Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase
基因别名: AMan II; GOLIM7; MAN2A1; Mana-2; MANA2; MANII; Manno; Map-2
UniProt ID: (Human) Q16706, (Mouse) P27046, (Rat) P28494
Entrez Gene ID: (Human) 4124, (Mouse) 17158, (Rat) 25478
mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity
zinc ion binding
hydrolase activity, hydrolyzing N-glycosyl compounds
carbohydrate binding
catalytic activity
hydrolase activity, hydrolyzing O-glycosyl compounds
alpha-mannosidase activity
mannosidase activity
hydrolase activity
hydrolase activity, acting on glycosyl bonds
metal ion binding
in utero embryonic development
liver development
carbohydrate metabolic process
mannose metabolic process
N-glycan processing
mitochondrion organization
vacuole organization
respiratory gaseous exchange
metabolic process
lung alveolus development
positive regulation of neurogenesis
retina morphogenesis in camera-type eye
protein glycosylation
protein deglycosylation
