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MARCH8 Antibody (PA5-88893) in WB
Western blot analysis of MARCH8 in extracts of various cell lines. Samples were incubated with MARCH8 Polyclonal antibody (Product # PA5-88893) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
产品信息
PA5-88893
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 232-291 of human MARCH8 (NP_6594582)
偶联物
形式
Liquid
浓度
0.37 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2805201
产品详细信息
Immunogen sequence: YNRVIYVQNC PETSKKNIFE KSPLTEPNFE NKHGYGICHS DTNSSCCTEP EDTGAEIIHV; Positive Samples: 22Rv1, U-87MG, Mouse testis, Rat testis, Rat liver; Cellular Location: Cytoplasmic vesicle membrane, Early endosome membrane, Lysosome membrane, Multi-pass membrane protein
靶标信息
MARCH8 (c-MIR) is a novel E3 ubiquitin ligase designated as the modulator of immune recognition (MIR) family, whose catalytic domain is a variant RING domain (RING-CH domain). MARCH8 was found as a functional and structural homolog of KSHV MIR1 and MIR2. MARCH8 targets B7-2 to lysosomal degradation and down-regulates the B7-2 surface expression through ubiquitination of its cytoplasmic tail. Furthermore, MARCH8 has been shown to down-regulate the expression of transferrin receptor and Fas, an important molecule for the induction of apoptosis. MARCH8 is the first example of an E3 ubiquitin ligase that is capable of inhibiting MHC II expression. Recent findings suggest that MARCH8 may regulate immune responses by promoting ubiquitination of MHC-II and CD86, leading to their subsequent endocytosis and lysosomal degradation.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: c-MIR; c-mir, cellular modulator of immune recognition; Cellular modulator of immune recognition; cellular modulator of immune recognition (c-MIR); E3 ubiquitin-protein ligase MARCH8; E3 ubiquitin-protein ligase MARCHF8; MARCH-VIII; membrane associated ring finger 8; membrane-associated ring finger (C3HC4) 8, E3 ubiquitin protein ligase; Membrane-associated RING finger protein 8; Membrane-associated RING-CH protein VIII; RING finger protein 178; RING-type E3 ubiquitin transferase MARCHF8; RP11-67C2.1
基因别名: 1300017E09Rik; c-MIR; CMIR; MARCH-VIII; MARCH8; MARCHF8; MIR; RNF178
UniProt ID: (Human) Q5T0T0, (Mouse) Q9DBD2
Entrez Gene ID: (Human) 220972, (Mouse) 71779, (Rat) 312656
