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MARCO Antibody (25-5447-42) in Flow
Human monocyte derived macrophages were stimulated with either IFNg and LPS (left) or IL-10 (right) for 48 hours, and subsequently stained with CD11b Monoclonal Antibody, FITC (Product # 11-0112-82) and MARCO Monoclonal Antibody, PE-Cyanine7. Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).
产品信息
25-5447-42
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG3
分类
Monoclonal
类型
Antibody
克隆号
PLK-1
抗原
Human alveolar macrophages
偶联物
激发/发射光谱
569/780 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2762452
产品详细信息
Description: This PLK-1 monoclonal antibody recognizes human MARCO also known as SR-A6 or SCARA2.
Applications Reported: This PLK-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This PLK-1 antibody has been pre-diluted and tested by flow cytometric analysis of human monocyte-derived macrophages. This may be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-34) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
靶标信息
MARCO, also known as SR-A6 or SCARA2, is a macrophage surface receptor and a member of the class A scavenger receptor family. As a part of the innate antimicrobial immune system, MARCO can bind both Gram-negative and Gram-positive bacteria via an extracellular, C-terminal, scavenger receptor cysteine-rich (SRCR) domain. In addition to short cytoplasmic and transmembrane domains, there is an extracellular spacer domain and a long, extracellular collagenous domain. The protein may form a trimeric molecule by the association of the collagenous domains of three identical polypeptide chains. Due to its expression in tumor infiltrating macrophages, and its role in inhibiting immune responses within tumor environment, MARCO has been indicated as a potential target for cancer immunotherapy.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: Macrophage receptor MARCO; Macrophage receptor with collagenous structure; Scavenger receptor class A member 2; scavenger receptor class A, member 2; SR-A6
基因别名: MARCO; SCARA2
UniProt ID: (Human) Q9UEW3
Entrez Gene ID: (Human) 8685
